Flotillin 1 Recombinant monoclonal antibody 83598-4-RR proteintech
$149.00
In stock
SKU
83598-4-RR
| Catalog Number | 83598-4-RR |
| Synonyms | FLOT1, 240582G6, Flotillin-1 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | U-87 MG cells, U-251 cells, LNCaP cells, Raji cells, mouse heart tissue, mouse brain tissue, rat brain tissue |
| Tested Applications — Positive IP detected in | mouse brain tissue |
| Tested Applications — Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HeLa cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| Positive WB detected in | U-87 MG cells, U-251 cells, LNCaP cells, Raji cells, mouse heart tissue, mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Ag7902 Product name: Recombinant human Flotillin 1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 80-427 aa of BC001146 Sequence: MLAAACQMFLGKTEAEIAHIALETLEGHQRAIMAHMTVEEIYKDRQKFSEQVFKVASSDLVNMGISVVSYTLKDIHDDQDYLHSLGKARTAQVQKDARIGEAEAKRDAGIREAKAKQEKVSAQYLSEIEMAKAQRDYELKKAAYDIEVNTRRAQADLAYQLQVAKTKQQIEEQRVQVQVVERAQQVAVQEQEIARREKELEARVRKPAEAERYKLERLAEAEKSQLIMQAEAEAASVRMRGEAEAFAIGARARAEAEQMAKKAEAFQLYQEAAQLDMLLEKLPQVAEEISGPLTSANKITLVSSGSGTMGAAKVTGEVLDILTRLPESVERLTGVSISQVNHKPLRTA Predict reactive species |
| Full Name | flotillin 1 |
| Calculated Molecular Weight | 47 kDa |
| Observed Molecular Weight | 47 kDa |
| GenBank Accession Number | BC001146 |
| Gene Symbol | Flotillin 1 |
| Gene ID (NCBI) | 10211 |
| RRID | AB_3671213 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purfication |
| UNIPROT ID | O75955 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for Flotillin 1 antibody 83598-4-RR | Download protocol |
| IHC protocol for Flotillin 1 antibody 83598-4-RR | Download protocol |
| IP protocol for Flotillin 1 antibody 83598-4-RR | Download protocol |
| WB protocol for Flotillin 1 antibody 83598-4-RR | Download protocol |
| mouse | WB J Neurochem Young Regulatory T Cell-Derived Extracellular Vesicles Improve Mitochondrial Function and Angiogenesis and Suppress Inflammation in Senescent Brain and Heart. Authors - Jiahui Cheng View Article |
| Citations | 1 |
| Dilutions | WB : 1:5000-1:50000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:200-1:800 IF/ICC : 1:250-1:1000 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 240582G6 |
| Conjugation | Unconjugated |
Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins commonly used as lipid raft markers. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth. Protocols Product Specific Protocols IF protocol for Flotillin 1 antibody 83598-4-RR Download protocol IHC protocol for Flotillin 1 antibody 83598-4-RR Download protocol IP protocol for Flotillin 1 antibody 83598-4-RR Download protocol WB protocol for Flotillin 1 antibody 83598-4-RR Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Young Regulatory T Cell-Derived Extracellular Vesicles Improve Mitochondrial Function and Angiogenesis and Suppress Inflammation in Senescent Brain and Heart.
Journal: J Neurochem | Authors: Jiahui Cheng | Species: mouse | Application: WB
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