HSL Recombinant monoclonal antibody 84540-1-RR proteintech
$149.00
In stock
SKU
84540-1-RR
| Catalog Number | 84540-1-RR |
| Synonyms | LIPE, LHS, Hormone-sensitive lipase, EC:3.1.1.79, EC:3.1.1.23 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | human testis tissue, mouse adipose tissue, rat adipose tissue |
| Tested Applications — Positive IHC detected in | mouse testis tissue, mouse brown adipose tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Positive WB detected in | human testis tissue, mouse adipose tissue, rat adipose tissue |
| Positive IHC detected in | mouse testis tissue, mouse brown adipose tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Tested Reactivity | human, mouse, rat |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Ag11360 Product name: Recombinant human LIPE protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 728-1076 aa of BC070041 Sequence: GNLCFTVALRAAAYGVRVPDGIMAAYPATMLQPAASPSRLLSLMDPLLPLSVLSKCVSAYAGAKTEDHSNSDQKALGMMGLVRRDTALLLRDFRLGASSWLNSFLELSGRKSQKMSEPIAEPMRRSVSEAALAQPQGPLGTDSLKNLTLRDLSLRGNSETSSDTPEMSLSAETLSPSTPSDVNFLLPPEDAGEEAEAKNELSPMDRGLGVRAAFPEGFHPRRSSQGATQMPLYSSPIVKNPFMSPLLAPDSMLKSLPPVHIVACALDPMLDDSVMLARRLRNLGQPVTLRVVEDLPHGFLTLAALCRETRQAAELCVERIRLVLTPPAGAGPSGETGAAGVDGGCGGRH Predict reactive species |
| Full Name | lipase, hormone-sensitive |
| Calculated Molecular Weight | 117 kDa |
| Observed Molecular Weight | 84 kDa |
| GenBank Accession Number | BC070041 |
| Gene Symbol | HSL |
| Gene ID (NCBI) | 3991 |
| RRID | AB_3672045 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | Q05469 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for HSL antibody 84540-1-RR | Download protocol |
| IHC protocol for HSL antibody 84540-1-RR | Download protocol |
| WB protocol for HSL antibody 84540-1-RR | Download protocol |
| Citations | - |
| Dilutions | WB : 1:5000-1:50000 IHC : 1:200-1:800 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 241875B11 |
| Conjugation | Unconjugated |
Hormone sensitive lipase (HSL) belongs to the 'GDXG' lipolytic enzyme family. In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production. HSL has two isoforms with the molecular mass of 117 kDa and 84 kDa. Protocols Product Specific Protocols IF protocol for HSL antibody 84540-1-RR Download protocol IHC protocol for HSL antibody 84540-1-RR Download protocol WB protocol for HSL antibody 84540-1-RR Download protocol Standard Protocols Click here to view our Standard Protocols
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