HUS1 Polyclonal antibody 11223-1-AP proteintech
$149.00
In stock
SKU
11223-1-AP
| Catalog Number | 11223-1-AP |
| Synonyms | Checkpoint protein HUS1, hHUS1, HUS1 |
| Host | Rabbit |
| Reactivity | human |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, ELISA |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | BxPC-3 cells |
| Tested Applications — Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:300-1:1000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Positive WB detected in | BxPC-3 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:300-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag1744 Product name: Recombinant human HUS1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-280 aa of BC007013 Sequence: MKFRAKIVDGACLNHFTRISNMIAKLAKTCTLRISPDKLNFILCDKLANGGVSMWCELEQENFFNEFQMEGVSAENNEIYLELTSENLSRALKTAQNARALKIKLTNKHFPCLTVSVELLSMSSSSRIVTHDIPIKVIPRKLWKDLQEPVVPDPDVSIYLPVLKTMKSVVEKMKNISNHLVIEANLDGELNLKIETELVCVTTHFKDLGNPPLASESTHEDRNVEHMAEVHIDIRKLLQFLAGQQVNPTKALCNIVNNKMVHFDLLHEDVSLQYFIPALS Predict reactive species |
| Full Name | HUS1 checkpoint homolog (S. pombe) |
| Calculated Molecular Weight | 32 kDa |
| Observed Molecular Weight | 32 kDa |
| GenBank Accession Number | BC007013 |
| Gene Symbol | HUS1 |
| Gene ID (NCBI) | 3364 |
| RRID | AB_2248839 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60921 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for HUS1 antibody 11223-1-AP | Download protocol |
| WB protocol for HUS1 antibody 11223-1-AP | Download protocol |
| human | WB Dis Markers Discovery and Validation of Novel Methylation Markers in Helicobacter pylori-Associated Gastric Cancer. Authors - Huan Wang View Article |
| Citations | 7 |
| Dilutions | WB : 1:300-1:1000 IHC : 1:20-1:200 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Hus1 belongs to the Rad family, form a heterotrimeric clamp with Rad1and Rad9 that acts as a putative sensor for DNA damage. This complex plays an apical role in the DNA damage response, loss of a functional complex affects many downstream checkpoint pathways [PMID: 11340080]. It is also a key complex involved in the activation of the ATR pathway. 9-1-1 forms a ring-shaped heterotrimer, resembling the homotrimeric sliding clamp, proliferating cell nuclear antigen (PCNA) [PMID: 19535328]. Protocols Product Specific Protocols IHC protocol for HUS1 antibody 11223-1-AP Download protocol WB protocol for HUS1 antibody 11223-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Uncoupling DNA damage from chromatin damage to detoxify doxorubicin.
Journal: Proc Natl Acad Sci U S A | Authors: Xiaohang Qiao | Application: WB - DNA damage-induced translocation of mitochondrial factor HIGD1A into the nucleus regulates homologous recombination and radio/chemo-sensitivity.
Journal: Oncogene | Authors: Bin Chen | Species: human | Application: WB - SUMO-1 modification of FEN1 facilitates its interaction with Rad9-Rad1-Hus1 to counteract DNA replication stress.
Journal: J Mol Cell Biol | Authors: Xiaoli Xu | Species: human | Application: WB - HUS1 checkpoint clamp component (HUS1) is a potential tumor suppressor in primary hepatocellular carcinoma.
Journal: Mol Carcinog | Authors: Zi-Qi Zhou KD Validated | Species: human | Application: WB,IHC - Succinylation at a key residue of FEN1 is involved in the DNA damage response to maintain genome stability.
Journal: Am J Physiol Cell Physiol | Authors: Rongyi Shi | Species: human | Application: IF - Discovery and Validation of Novel Methylation Markers in Helicobacter pylori-Associated Gastric Cancer.
Journal: Dis Markers | Authors: Huan Wang | Species: human | Application: WB
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