MAVS; VISA Recombinant monoclonal antibody 81910-1-RR proteintech
$149.00
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SKU
81910-1-RR
| Catalog Number | 81910-1-RR |
| Synonyms | MAVS, Cardif, IPS 1, IPS1, IPS-1 |
| Host | Rabbit |
| Reactivity | human and More (1) |
| Reactivity | human, pig |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IP, CoIP, ELISA |
| Applications | WB, IHC, IF/ICC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HeLa cells, HepG2 cells, A431 cells, A549 cells, MCF-7 cells |
| Tested Applications — Positive IP detected in | HEK-293 cells |
| Tested Applications — Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HeLa cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Positive WB detected in | HeLa cells, HepG2 cells, A431 cells, A549 cells, MCF-7 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Tested Reactivity | human |
| Cited Reactivity | human, pig |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Ag5655 Product name: Recombinant human MAVS; VISA protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-347 aa of BC044952 Sequence: MPFAEDKTYKYICRNFSNFCNVDVVEILPYLPCLTARDQDRLRATCTLSGNRDTLWHLFNTLQRRPGWVEYFIAALRGCELVDLADEVASVYESYQPRTSDRPPDPLEPPSLPAERPGPPTPAAAHSIPYNSCREKEPSYPMPVQETQAPESPGENSEQALQTLSPRAIPRNPDGGPLESSSDLAALSPLTSSGHQEKDTELGSTHTAGATSSLTPSRGPVSPSVSFQPLARSTPRASRLPGPTGSVVSTGTSFSSSSPGLASAGAAEGKQGAESDQAEPIICSSGAEAPANSLPSKVPTTLMPVNTVALKVPANPASVSTVPSKLPTSSKPPGAVPSNALTNPAPS Predict reactive species |
| Full Name | mitochondrial antiviral signaling protein |
| Calculated Molecular Weight | 57 kDa |
| Observed Molecular Weight | 75 kDa |
| GenBank Accession Number | BC044952 |
| Gene Symbol | MAVS |
| Gene ID (NCBI) | 57506 |
| RRID | AB_2935592 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | Q7Z434 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for MAVS; VISA antibody 81910-1-RR | Download protocol |
| IHC protocol for MAVS; VISA antibody 81910-1-RR | Download protocol |
| IP protocol for MAVS; VISA antibody 81910-1-RR | Download protocol |
| WB protocol for MAVS; VISA antibody 81910-1-RR | Download protocol |
| human | WB,IF Virulence 3C suppresses PINK1-mediated mitophagy and contributes to coxsackievirus B3 replication. Authors - Tingjun Liu View Article |
| pig | WB,IF,CoIP Vet Microbiol ASFV early protein p30 suppresses antiviral type I IFN induction by targeting TRIM21 and RIG-I like receptor signaling adaptor MAVS. Authors - Jiajia Zhang View Article |
| Citations | 3 |
| Dilutions | WB : 1:5000-1:50000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:500-1:2000 IF/ICC : 1:500-1:2000 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 7F14 |
| Conjugation | Unconjugated |
Mitochondrial antiviral-signaling protein (MAVS) is also known as virus-induced-signaling adapter (VISA) or IFN-β promoter stimulator protein 1 (IPS-1), it is widely involved and required for innate immune defense against viruses. MAVS, present in T cells, monocytes, epithelial cells and hepatocytes, contains CARD and transmembrane domains which are essential for antiviral functions. MAVS is able to interact with various cellular proteins including DDX58/RIG-I, IFIH1/MDA5, TRAF2, TRAF6, TMEM173/MITA, IFIT3 and etc. It can undergoe phosphorylation on multiple sites and ubiquitination, which may together cause the molecular weight migrate to about 70 kDa despite the predicated 57 kDa. Protocols Product Specific Protocols IF protocol for MAVS; VISA antibody 81910-1-RR Download protocol IHC protocol for MAVS; VISA antibody 81910-1-RR Download protocol IP protocol for MAVS; VISA antibody 81910-1-RR Download protocol WB protocol for MAVS; VISA antibody 81910-1-RR Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- 3C suppresses PINK1-mediated mitophagy and contributes to coxsackievirus B3 replication.
Journal: Virulence | Authors: Tingjun Liu | Species: human | Application: WB,IF - Topical Glycolysis Inhibition Restores MAVS-Associated Antiviral Signaling in Herpes Simplex Keratitis.
Journal: Invest Ophthalmol Vis Sci | Authors: Wei Xu - ASFV early protein p30 suppresses antiviral type I IFN induction by targeting TRIM21 and RIG-I like receptor signaling adaptor MAVS.
Journal: Vet Microbiol | Authors: Jiajia Zhang | Species: pig | Application: WB,IF,CoIP
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