| Catalog Number | 85043-1-RR |
| Synonyms | 242170F4, AOFMD, AVMD, Peripherin 2, Peripherin2 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF-P, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | mouse eye tissue, rat eye tissue |
| Tested Applications — Positive IHC detected in | mouse eye tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF-P detected in | mouse eye tissue |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Recommended Dilutions — Immunofluorescence (IF)-P | IF-P : 1:400-1:1600 |
| Positive WB detected in | mouse eye tissue, rat eye tissue |
| Positive IHC detected in | mouse eye tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse eye tissue |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-P | IF-P : 1:400-1:1600 |
| Tested Reactivity | human, mouse, rat |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Ag12555 Product name: Recombinant human PRPH2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 124-346 aa of BC074720 Sequence: GSLENTLGQGLKNGMKYYRDTDTPGRCFMKKTIDMLQIEFKCCGNNGFRDWFEIQWISNRYLDFSSKEVKDRIKSNVDGRYLVDGVPFSCCNPSSPRPCIQYQITNNSAHYSYDHQTEELNLWVRGCRAALLSYYSSLMNSMGVVTLLIWLFEVTITIGLRYLQTSLDGVSNPEESESESEGWLLEKSVPETWKAFLESVKKLGKGNQVEAEGAGAGQAPEAG Predict reactive species |
| Full Name | peripherin 2 (retinal degeneration, slow) |
| Calculated Molecular Weight | 346 aa, 39 kDa |
| Observed Molecular Weight | 30-40 kDa |
| GenBank Accession Number | BC074720 |
| Gene Symbol | PRPH2 |
| Gene ID (NCBI) | 5961 |
| RRID | AB_3743963 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purfication |
| UNIPROT ID | P23942 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for PRPH2 antibody 85043-1-RR | Download protocol |
| IHC protocol for PRPH2 antibody 85043-1-RR | Download protocol |
| WB protocol for PRPH2 antibody 85043-1-RR | Download protocol |
| Citations | - |
| Dilutions | WB : 1:5000-1:50000 IHC : 1:200-1:800 IF-P : 1:400-1:1600 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 242170F4 |
| Conjugation | Unconjugated |
Peripherin-2 (PRPH2), also known as retinal degeneration slow protein (RDS), is a photoreceptor-specific tetraspanin protein implicated in outer segment disk morphogenesis. It may function as an adhesion molecule involved in the stabilization and compaction of outer segment disks or in the maintenance of the curvature of the rim. Mutations in peripherin-2 are responsible for various retinal degenerative diseases including autosomal dominant retinitis pigmentosa (ADRP). Protocols Product Specific Protocols IF protocol for PRPH2 antibody 85043-1-RR Download protocol IHC protocol for PRPH2 antibody 85043-1-RR Download protocol WB protocol for PRPH2 antibody 85043-1-RR Download protocol Standard Protocols Click here to view our Standard Protocols