TIA1 Recombinant monoclonal antibody 82672-1-RR proteintech
$149.00
In stock
SKU
82672-1-RR
| Catalog Number | 82672-1-RR |
| Synonyms | Cytotoxic granule associated RNA binding protein TIA1, Nucleolysin TIA-1 isoform p40, p40 TIA 1, p40-TIA-1, RNA binding protein TIA 1 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | Caco-2 cells, Jurkat cells, COLO 320 cells, K-562 cells, MOLT-4 cells, NIH/3T3 cells, HSC-T6 cells |
| Tested Applications — Positive IF/ICC detected in | sodium arsenite treated HeLa cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Positive WB detected in | Caco-2 cells, Jurkat cells, COLO 320 cells, K-562 cells, MOLT-4 cells, NIH/3T3 cells, HSC-T6 cells |
| Positive IF/ICC detected in | sodium arsenite treated HeLa cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Tested Reactivity | human, mouse, rat |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Ag2778 Product name: Recombinant human TIA1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-214 aa of BC015944 Sequence: MEDEMPKTLYVGNLSRDVTEALILQLFSQIGPCKNCKMIMDTAGNDPYCFVEFHEHRHAAAALAAMNGRKIMGKEVKVNWATTPSSQKKDTSSSTVVSTQRSQDHFHVFVGDLSPEITTEDIKAAFAPFGRISDARVVKDMATGKSKGYGFVSFFNKWDAENAIQQMGGQWLGGRQIRTNWATRKPPAPKSTYECRCIGEEKEMWNFGEKYARF Predict reactive species |
| Full Name | TIA1 cytotoxic granule-associated RNA binding protein |
| Calculated Molecular Weight | 214 aa, 24 kDa, 43 kDa |
| Observed Molecular Weight | 38-40 kDa |
| GenBank Accession Number | BC015944 |
| Gene Symbol | TIA1 |
| Gene ID (NCBI) | 7072 |
| RRID | AB_3086500 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | P31483 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for TIA1 antibody 82672-1-RR | Download protocol |
| WB protocol for TIA1 antibody 82672-1-RR | Download protocol |
| Citations | - |
| Dilutions | WB : 1:5000-1:50000 IF/ICC : 1:500-1:2000 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 3N22 |
| Conjugation | Unconjugated |
TIA1, also named as p40-TIA-1, is involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). It possesses nucleolytic activity against cytotoxic lymphocyte target cells. TIA1 may be involved in apoptosis. Two isoforms of this protein exist - 41kDa and 42kDa. one of these was a missense variant (P362L) in TIA1. Similar to the ALS-related disease proteins TDP-43, hnRNPA1, and FUS, TIA1 is an RNA-binding protein containing a prionlike LCD and assembles into membrane-less organelles, including SGs. Postmortem neuropathology of five TIA1 mutations carriers showed a consistent pathological signature with numerous round, hyaline, TAR DNA-binding protein 43 (TDP-43)-positive inclusions.TIA1mutations significantly increased the propensity of TIA1 protein to undergo phase transition. In live cells,TIA1mutations delayed stress granule (SG) disassembly and promoted the accumulation of non-dynamic SGs that harbored TDP-43. Protocols Product Specific Protocols IF protocol for TIA1 antibody 82672-1-RR Download protocol WB protocol for TIA1 antibody 82672-1-RR Download protocol Standard Protocols Click here to view our Standard Protocols
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