Human LATS1 ELISA Kit KE01060 proteintech
$299.00
In stock
SKU
KE01060
| Product name | Human LATS1 ELISA Kit |
| Synonyms | EC:2.7.11.1, h-warts, LATS 1, Serine/threonine-protein kinase LATS1, WARTS |
| Reactivity | Human |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 2.2 pg/mL |
| Range | 39-2500 pg/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 9113 |
| Sample Type | Average Range |
| Cell lysate | 118% 89%-128% |
| Sample | n mean ( pg/mL ) SD CV% |
| 1 | 16 1,295.8 103.5 8.0 |
| 2 | 16 318.0 22.1 6.9 |
| 3 | 16 167.3 11.8 7.1 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
LATS1(Large tumor suppressor homolog 1) is also named as WARTS and belongs to the AGC Ser/Thr protein kinase family. The gene encodes a highly conserved (from fly to human) protein kinase that plays a crucial role in the prevention of tumor formation by controlling the progression of mitosis. The expression of both long(170 kDa) and short lats1 isoforms(120 kDa) in vertebrate retinal cells raises the possibility that these lats1 proteins may act as negative key regulators of the cell cycle each of them performing a unique role. In mammalian cells, LATS1 was phosphorylated in a cell cycle-dependent manner and complexed with CDC2 in early mitosis. LATS1 also play a key role in the regulation of Hippo pathway. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms EC:2.7.11.1, h-warts, LATS 1, Serine/threonine-protein kinase LATS1, WARTS FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
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