Speedy™ Human LECT2 One-Step ELISA Kit SE50240 proteintech
$449.00
In stock
SKU
SE50240
| Product name | Speedy™ Human LECT2 One-Step ELISA Kit |
| Synonyms | hLECT2, LECT 2, LECT-2, Leukocyte cell-derived chemotaxin-2 |
| Reactivity | Human |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 4.8 pg/mL |
| Range | 31.25-2000 pg/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3950 |
| Sample Type | Average Range |
| Human serum | 85% 73%-101% |
| Sample | n mean ( pg/mL ) SD CV% |
| 1 | 16 947.0 21.7 2.3 |
| 2 | 16 224.8 3.7 1.6 |
| 3 | 16 121.6 3.9 3.2 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
Leukocyte cell-derived chemotaxin 2 (LECT2) is a multi-functional protein primarily expressed in the liver. It belongs to the M23 metalloprotease family and acts as a hepatokine, adipokine, and chemotactic factor. LECT2 plays significant roles in various physiological and pathological processes, including immune regulation, inflammation, liver regeneration, and energy metabolism. Crucially, it has been identified as a functional ligand for the receptor CD209a, linking it to metabolic inflammation. Dysregulation of LECT2 is associated with several diseases, most notably as a key driver in the pathogenesis of amyloidosis (ALect2), where it misfolds and deposits in tissues, particularly the kidneys and liver. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms hLECT2, LECT 2, LECT-2, Leukocyte cell-derived chemotaxin-2 FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent. 2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data? Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.
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