| Product name | Human MEF2D ELISA Kit |
| Synonyms | Myocyte-specific enhancer factor 2D |
| Reactivity | Human |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.05 ng/mL |
| Range | 0.156-10 ng/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4209 |
| Sample Type | Average Range |
| Cell lysate | 98% 94%-101% |
| Sample | n mean ( ng/mL ) SD CV% |
| 1 | 16 4.92 0.23 4.67 |
| 2 | 16 1.41 0.10 7.09 |
| 3 | 16 0.81 0.05 6.17 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
Myocyte-specific enhancer factor 2D (MEF2D) is a transcription factor belonging to the MEF2 family, which plays critical roles in regulating gene expression during muscle development, neuronal differentiation, and immune cell function. MEF2D binds to specific DNA sequences in the promoter regions of target genes and interacts with various cofactors, including histone deacetylases and myogenic regulatory factors, to modulate transcriptional activity. Dysregulation of MEF2D has been implicated in several pathological conditions, including cancer, where it functions as either an oncogene or tumor suppressor depending on cellular context. In B-cell acute lymphoblastic leukemia (B-ALL), chromosomal translocations involving MEF2D lead to fusion proteins that drive leukemogenesis. Additionally, MEF2D contributes to neurodegenerative diseases through its role in neuronal survival and apoptosis. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms Myocyte-specific enhancer factor 2D FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.