CD45 Recombinant monoclonal antibody 80297-1-RR proteintech
$149.00
In stock
SKU
80297-1-RR
| Catalog Number | 80297-1-RR |
| Synonyms | EC:3.1.3.48, GP180, L CA, LCA, L-CA |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | Daudi cells, Raji cells, Ramos cells |
| Tested Applications — Positive IHC detected in | mouse colon tissue, human appendicitis tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF-P detected in | mouse spleen tissue |
| Tested Applications — Positive IF-Fro detected in | mouse colon tissue, mouse spleen tissue, Jurkat cells |
| Tested Applications — Positive IF/ICC detected in | Jurkat cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:20000-1:100000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:4000-1:16000 |
| Recommended Dilutions — Immunofluorescence (IF)-P | IF-P : 1:250-1:1000 |
| Recommended Dilutions — Immunofluorescence (IF)-FRO | IF-FRO : 1:125-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Positive WB detected in | Daudi cells, Raji cells, Ramos cells |
| Positive IHC detected in | mouse colon tissue, human appendicitis tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse spleen tissue |
| Positive IF-Fro detected in | mouse colon tissue, mouse spleen tissue, Jurkat cells |
| Positive IF/ICC detected in | Jurkat cells |
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunohistochemistry (IHC) | IHC : 1:4000-1:16000 |
| Immunofluorescence (IF)-P | IF-P : 1:250-1:1000 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:125-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Ag13712 Product name: Recombinant human CD45 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 550-750 aa of BC014239 Sequence: KIYDLHKKRSCNLDEQQELVERDDEKQLMNVEPIHADILLETYKRKIADEGRLFLAEFQSIPRVFSKFPIKEARKPFNQNKNRYVDILPYDYNRVELSEINGDAGSNYINASYIDGFKEPRKYIAAQGPRDETVDDFWRMIWEQKATVIVMVTRCEEGNRNKCAEYWPSMEEGTRAFGDVVVKINQHKRCPDYIIQKLNIV Predict reactive species |
| Full Name | protein tyrosine phosphatase, receptor type, C |
| Calculated Molecular Weight | 1304 aa, 147 kDa |
| Observed Molecular Weight | 220-240 kDa |
| GenBank Accession Number | BC014239 |
| Gene Symbol | CD45 |
| Gene ID (NCBI) | 5788 |
| ENSEMBL Gene ID | ENSG00000081237 |
| RRID | AB_2918884 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | P08575 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for CD45 antibody 80297-1-RR | Download protocol |
| IHC protocol for CD45 antibody 80297-1-RR | Download protocol |
| WB protocol for CD45 antibody 80297-1-RR | Download protocol |
| human | IF Commun Biol An integrated map of fibroblastic populations in human colon mucosa and cancer tissues Authors - Siying Li View Article |
| mouse | IHC Sci Rep Heterozygous interferon signaling deficient mice as animal models for Chikungunya virus infection in the heart Authors - Shazeed-Ul Karim View Article |
| Kenzo (Verified Customer) (08-14-2023) | This antibody works well to immunolabel hematopoietic cells in mouse lymph nodes. Applications: Immunohistochemistry Primary Antibody Dilution: 1:200 Cell Tissue Type: Mouse lymph node |
| Citations | 6 |
| Dilutions | WB : 1:20000-1:100000 IHC : 1:4000-1:16000 IF-P : 1:250-1:1000 IF-FRO : 1:125-1:500 IF/ICC : 1:500-1:2000 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 6O19 |
| Conjugation | Unconjugated |
CD45, also known as leukocyte common antigen (LCA), B220, and T200, is a 180-240 kD single chain type I membrane glycoprotein. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 is one of the best negative selection markers for characterizing and/or isolating human mesenchymal stem cells from bone marrow and other sources. Along with the positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification. Protocols Product Specific Protocols IF protocol for CD45 antibody 80297-1-RR Download protocol IHC protocol for CD45 antibody 80297-1-RR Download protocol WB protocol for CD45 antibody 80297-1-RR Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Targeted Intracellular Copper Reservoir Enhances Liver Cancer Immunotherapy
Journal: Small | Authors: Tianao Xie | Species: human | Application: IF - CCL3 and LPS combination therapy significantly reduces infection and stimulates wound healing in diabetic mice by boosting proinflammatory responses.
Journal: J Invest Dermatol | Authors: Rajalekshmy G Padmakumari - Heterozygous interferon signaling deficient mice as animal models for Chikungunya virus infection in the heart
Journal: Sci Rep | Authors: Shazeed-Ul Karim | Species: mouse | Application: IHC - Molecular dynamics driving phenotypic divergence among KRAS mutants in pancreatic tumorigenesis.
Journal: Dev Cell | Authors: Adrien Grimont - An integrated map of fibroblastic populations in human colon mucosa and cancer tissues
Journal: Commun Biol | Authors: Siying Li | Species: human | Application: IF - Low-dose radiation ameliorates doxorubicin-induced renal injury via reducing oxidative stress and protecting mitochondrial function
Journal: PLoS One | Authors: Mengmeng Chen
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