Beta Catenin Recombinant monoclonal antibody 80488-1-RR proteintech
$149.00
In stock
SKU
80488-1-RR
| Catalog Number | 80488-1-RR |
| Synonyms | b cat, B-catenin, beta-Catenin, Catenin beta 1, Catenin beta-1 |
| Host | Rabbit |
| Reactivity | human, mouse, rat, pig, zebrafish |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IF-P, CoIP, ELISA, ChIP-qPCR |
| Applications | WB, IHC, IF/ICC, IF-P, ELISA, ChIP-qPCR |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | A431 cells, zebrafish tissue, HeLa cells, HEK-293 cells, MCF-7 cells, LNCaP cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, pig brain tissue |
| Tested Applications — Positive IHC detected in | human liver cancer tissue, human breast cancer tissue, human colon cancer tissue, human rectal cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF-P detected in | mouse colon tissue, rat pancreas tissue |
| Tested Applications — Positive IF/ICC detected in | MCF-7 cells |
| Tested Applications — Positive ChIP-qPCR detected in | HCT 116 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Recommended Dilutions — Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:1000-1:4000 |
| Positive WB detected in | A431 cells, zebrafish tissue, HeLa cells, HEK-293 cells, MCF-7 cells, LNCaP cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, pig brain tissue |
| Positive IHC detected in | human liver cancer tissue, human breast cancer tissue, human colon cancer tissue, human rectal cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse colon tissue, rat pancreas tissue |
| Positive IF/ICC detected in | MCF-7 cells |
| Positive ChIP-qPCR detected in | HCT 116 cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:1000-1:4000 |
| CHIP-QPCR | CHIP-QPCR : 1:10-1:100 |
| Tested Reactivity | human, mouse, rat, pig, zebrafish |
| Cited Reactivity | human, mouse |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Peptide Predict reactive species |
| Full Name | catenin (cadherin-associated protein), beta 1, 88kDa |
| Calculated Molecular Weight | 781 aa, 86 kDa |
| Observed Molecular Weight | 92 kDa |
| GenBank Accession Number | BC058926 |
| Gene Symbol | Beta Catenin |
| Gene ID (NCBI) | 1499 |
| ENSEMBL Gene ID | ENSG00000168036 |
| RRID | AB_2918895 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | P35222 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for Beta Catenin antibody 80488-1-RR | Download protocol |
| IHC protocol for Beta Catenin antibody 80488-1-RR | Download protocol |
| WB protocol for Beta Catenin antibody 80488-1-RR | Download protocol |
| human | WB Discov Oncol miR-124-3p inhibits CRC proliferation, migration, and invasion by targeting ITGB1 Authors - Jing Li View Article |
| mouse,human | WB,ChIP J Pharmacol Exp Ther Alleviation of metabolic dysfunction-associated steatotic liver disease by silymarin is associated with maintaining mitochondrial homeostasis via regulation of optic atrophy 1. Authors - Ming-Qiang Liu View Article |
| mouse | IF Front Cell Infect Microbiol Brucella melitensis clinical isolate modulates osteoclast differentiation to drive pathological bone destruction in brucellar arthritis. Authors - Jinlei Chen View Article |
| Citations | 6 |
| Dilutions | WB : 1:5000-1:50000 IHC : 1:1000-1:4000 IF-P : 1:200-1:800 IF/ICC : 1:1000-1:4000 CHIP-QPCR : 1:10-1:100 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 2J24 |
| Conjugation | Unconjugated |
β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin is a 92-kDa protein, originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222) Protocols Product Specific Protocols IF protocol for Beta Catenin antibody 80488-1-RR Download protocol IHC protocol for Beta Catenin antibody 80488-1-RR Download protocol WB protocol for Beta Catenin antibody 80488-1-RR Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- M6A modification-mediated LIMA1 promotes the progression of hepatocellular carcinoma through the wnt-βcatenin/Hippo pathway
Journal: Cell Biol Toxicol | Authors: Chao Zhang | Species: human | Application: WB,IF,CoIP - Alleviation of metabolic dysfunction-associated steatotic liver disease by silymarin is associated with maintaining mitochondrial homeostasis via regulation of optic atrophy 1.
Journal: J Pharmacol Exp Ther | Authors: Ming-Qiang Liu | Species: mouse,human | Application: WB,ChIP - Brucella melitensis clinical isolate modulates osteoclast differentiation to drive pathological bone destruction in brucellar arthritis.
Journal: Front Cell Infect Microbiol | Authors: Jinlei Chen | Species: mouse | Application: IF - miR-124-3p inhibits CRC proliferation, migration, and invasion by targeting ITGB1
Journal: Discov Oncol | Authors: Jing Li | Species: human | Application: WB - FcγRIIB functions as an IgG transporter in the mammary gland.
Journal: Proc Natl Acad Sci U S A | Authors: Xin Li - Engineering multifunctional chitosan-based hydrogels for integrated antimicrobial therapy and endogenous stem cell-driven tissue repair in infectious keratitis.
Journal: Bioact Mater | Authors: Wen Geng
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