Phospho-AMPK Beta 1 (Ser182) Recombinant monoclonal antibody 83924-1-RR proteintech
$149.00
In stock
SKU
83924-1-RR
| Catalog Number | 83924-1-RR |
| Synonyms | AMPKβ1 (Ser182), AMPKβ1Ser182, p AMPK, p AMPK Beta 1, p AMPK Beta 1 (Ser182) |
| Host | Rabbit |
| Reactivity | human and More (3) |
| Reactivity | human, mouse, rat, pig |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, ELISA, Western Blot |
| Applications | WB, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HEK-293 cells, λ phosphatase treated HEK-293 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:1000 |
| Positive WB detected in | HEK-293 cells, λ phosphatase treated HEK-293 cells |
| Western Blot (WB) | WB : 1:500-1:1000 |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, pig |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Peptide Predict reactive species |
| Full Name | protein kinase, AMP-activated, beta 1 non-catalytic subunit |
| Calculated Molecular Weight | 38 kDa |
| Observed Molecular Weight | 38 kDa |
| GenBank Accession Number | BC001007 |
| Gene Symbol | PRKAB1 |
| Gene ID (NCBI) | 5564 |
| RRID | AB_3671504 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q9Y478 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| WB protocol for Phospho-AMPK Beta 1 (Ser182) antibody 83924-1-RR | Download protocol |
| pig | WB Front Nutr Luteolin attenuates LPS-induced damage in IPEC-J2 cells by enhancing mitophagy via AMPK signaling pathway activation Authors - Jianyun Yuan View Article |
| human | WB Anal Cell Pathol (Amst) Shikonin Induces Autophagy and Apoptosis in Esophageal Cancer EC9706 Cells by Regulating the AMPK/mTOR/ULK Axis Authors - Junli Zhang View Article |
| mouse | WB Clin Exp Pharmacol Physiol Gemfibrozil Prevents Myocardial Ischemia-Reperfusion Injury in Mice Through AMPK Activation. Authors - Yilong Jiang View Article |
| Citations | 29 |
| Dilutions | WB : 1:500-1:1000 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 240628B1 |
| Conjugation | Unconjugated |
AMPK Beta 1 (5'-AMP-activated protein kinase subunit beta-1) is also named as PRKAB1 and AMPK. AMPK, a serine/threonine kinase that exists as a heterotrimer comprised of a catalytic α-subunit and regulatory β- and γ-subunits, has been recognized as a sensor of cellular energy homeostasis (PMID: 21937710). AMPK regulates key metabolic enzymes, cell growth, apoptosis, gene transcription, and protein synthesis (PMID: 12829246). AMPK is an energy sensor and plays an essential role in the control of cellular bioenergetics by responding to various stresses including those that induce changes in the cellular AMP:ATP ratio or modulation in intracellular calcium (PMID: 27812976, PMID: 26616193). Recent studies have shown that AMPK mediates the inhibition of cell proliferation and growth of tumor cells (PMID: 16613876). AMPK also inhibits the expression of Glut1 and glycolysis in Tregs by inhibiting mTORC1 signaling (PMID: 25477880). This antibody recognizes phosphorylated AMPK Beta 1. Protocols Product Specific Protocols WB protocol for Phospho-AMPK Beta 1 (Ser182) antibody 83924-1-RR Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Collagen peptides from Skipjack tuna (Katsuwonus pelamis) bones: Composition, characterization, and mechanism of high-fat diet-induced NAFLD amelioration
Journal: Food Res Int | Authors: Yu-Lin Jiang - Luteolin attenuates LPS-induced damage in IPEC-J2 cells by enhancing mitophagy via AMPK signaling pathway activation
Journal: Front Nutr | Authors: Jianyun Yuan | Species: pig | Application: WB - Shikonin Induces Autophagy and Apoptosis in Esophageal Cancer EC9706 Cells by Regulating the AMPK/mTOR/ULK Axis
Journal: Anal Cell Pathol (Amst) | Authors: Junli Zhang | Species: human | Application: WB - Injectable SF-platform orchestrates GPX4-targeted ferroptosis-autophagy-immunogenic circuit for overcoming oxidative resistance in triple-negative breast cancer
Journal: Theranostics | Authors: Hui Yuan - Gemfibrozil Prevents Myocardial Ischemia-Reperfusion Injury in Mice Through AMPK Activation.
Journal: Clin Exp Pharmacol Physiol | Authors: Yilong Jiang | Species: mouse | Application: WB - Gastrodin Attenuates Cerebral Ischemia-Reperfusion Injury by Enhancing Mitochondrial Fusion and Activating the AMPK-OPA1 Signaling Pathway
Journal: CNS Neurosci Ther | Authors: Zihan Liu - Collagen peptides from Skipjack tuna (Katsuwonus pelamis) bones: Composition, characterization, and mechanism of high-fat diet-induced NAFLD amelioration
Journal: Food Res Int | Authors: Yu-Lin Jiang - Homoharringtonine inhibits growth and migration in non-small cell lung cancer via PDIA4-mediated modulation of autophagy and EMT.
Journal: Arch Pharm Res | Authors: Xiaoyu Ma | Species: human | Application: WB,ELISA - Shionone Inhibits Glomerular Fibirosis by Suppressing NLRP3 Related Inflammasome though SESN2-NRF2/ HO-1 Pathway
Journal: Diabetes Metab J | Authors: Tian Xiao | Species: mouse | Application: WB - Flavonoid-rich extracts of Nelumbo nucifera leaves alleviate obesity in HFD-fed mice via microbiota-dependent modulation of brown fat thermogenesis
Journal: J Ethnopharmacol | Authors: Zhenyu Wang | Species: mouse | Application: WB - Orally Administered Neohesperidin (Neo) Alleviates Lipopolysaccharide-Induced Mastitis by Suppressing TLR4/NF-κB and Activating AMPK/Nrf2/HO-1 Signaling, and Regulating Intestinal Flora Structure.
Journal: J Agric Food Chem | Authors: Dewei He | Species: mouse | Application: WB - Gastrodin Attenuates Cerebral Ischemia-Reperfusion Injury by Enhancing Mitochondrial Fusion and Activating the AMPK-OPA1 Signaling Pathway
Journal: CNS Neurosci Ther | Authors: Zihan Liu - Protective Effects of Isostrictiniin Against High-Fat, High-Sugar Diet-Induced Steatosis in MASLD Mice via Regulation of the AMPK/SREBP-1c/ACC Pathway
Journal: Nutrients | Authors: Qi Yan | Species: mouse | Application: WB - Unlocking Hopeaphenol: A Potent Ally Against Cardiac Hypertrophy via AMPK Activation.
Journal: Nutrients | Authors: Jinhong Chen | Species: mouse | Application: WB - Alisol B alleviates MASLD by activating liver autophagy and fatty acid oxidation via Ces2a
Journal: Int Immunopharmacol | Authors: Congcong Zhang | Species: mouse | Application: WB - Luteolin attenuates LPS-induced damage in IPEC-J2 cells by enhancing mitophagy via AMPK signaling pathway activation
Journal: Front Nutr | Authors: Jianyun Yuan | Species: pig | Application: WB - SRPK1 is a significant factor in driving the progression of diabetic kidney fibrosis.
Journal: Diabetol Metab Syndr | Authors: Shichao Han | Species: mouse | Application: IHC - Qula-derived Limosilactobacillus fermentum TD-3 and Lactococcus lactis MQ1-1 alleviate aging-related intestinal barrier dysfunction via microbiota-short-chain fatty acid-AMPK/MLCK-tight junction axis
Journal: J Dairy Sci | Authors: Xiaolin Liu | Species: human | Application: WB - Targeted therapy for KIF3C: A study on the mechanism of combined therapy with KIF3C signaling pathway, afatinib, and MT-DC (ac)phosphoramide in regulating gastric cancer cell proliferation
Journal: Cell Signal | Authors: Maladho Tanta Diallo | Species: human | Application: WB - miR-210 Regulates Autophagy Through the AMPK/mTOR Signaling Pathway, Reduces Neuronal Cell Death and Inflammatory Responses, and Enhances Functional Recovery Following Cerebral Hemorrhage in Mice
Journal: Neurochem Res | Authors: Yao Wang | Application: WB - Mitochondrial uncoupler BAM15 ameliorates liver lipid metabolism disorders by activating the AMPK pathway.
Journal: FEBS J | Authors: Zunhai Liu | Species: mouse | Application: WB - Hawthorn total flavonoids ameliorate hyperlipidemia through AMPK/SREBP1-c and PPARα/PGC-1α/CPT-1A pathway activation and gut microbiota modulation
Journal: J Sci Food Agric | Authors: Liping Zheng | Species: mouse | Application: WB - N-Lac-Phe Ameliorates Myocardial Cell Death by Improving Mitochondrial Dysfunction, Ultimately Improving TAC-induced Heart Failure.
Journal: Cardiovasc Drugs Ther | Authors: Lei Sha | Species: mouse | Application: WB - Metabolic reprogramming-driven stratification and therapeutic targeting in lung adenocarcinoma: implications for prognosis and personalized treatment.
Journal: Front Oncol | Authors: Jiabao Jia | Species: human | Application: WB - Hsa_circ_0006834 represses intrahepatic cholangiocarcinoma proliferation through activating AMPK-mTOR pathway and autophagy via has-miR-637-NGFR network
Journal: PLoS One | Authors: Ji Wang | Species: human | Application: WB - Metformin promotes mitochondrial integrity through AMPK-signaling in Leber's hereditary optic neuropathy.
Journal: FEBS Open Bio | Authors: Chatnapa Panusatid | Species: human | Application: IF - Shikonin Induces Autophagy and Apoptosis in Esophageal Cancer EC9706 Cells by Regulating the AMPK/mTOR/ULK Axis
Journal: Anal Cell Pathol (Amst) | Authors: Junli Zhang | Species: human | Application: WB - Gemfibrozil Prevents Myocardial Ischemia-Reperfusion Injury in Mice Through AMPK Activation.
Journal: Clin Exp Pharmacol Physiol | Authors: Yilong Jiang | Species: mouse | Application: WB - The highly expressed GOLPH3 in colorectal cancer cells activates smoothened to drive glycolysis and promote cancer cell growth and radiotherapy resistance
Journal: J Gastrointest Oncol | Authors: Kunli Zhu | Species: human | Application: WB
Product Documents
Reviews
Write Your Own Review