CD68 Recombinant monoclonal antibody 83014-5-RR proteintech
$149.00
In stock
SKU
83014-5-RR
| Catalog Number | 83014-5-RR |
| Synonyms | 230504G10, CD68 antigen, gp110, Scard1 |
| Host | Rabbit |
| Reactivity | mouse, rat and More (1) |
| Reactivity | mouse, rat, pig |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF-P, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | RAW 264.7 cells, J774A.1 cells, NR8383 cells |
| Tested Applications — Positive IHC detected in | mouse spleen tissue, mouse liver tissue, rat small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF-P detected in | mouse spleen tissue, rat small intestine tissue |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Recommended Dilutions — Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Positive WB detected in | RAW 264.7 cells, J774A.1 cells, NR8383 cells |
| Positive IHC detected in | mouse spleen tissue, mouse liver tissue, rat small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse spleen tissue, rat small intestine tissue |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Tested Reactivity | mouse, rat |
| Cited Reactivity | mouse, rat, pig |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Eg0787 Product name: Recombinant mouse CD68 protein Source: mammalian cells -derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 21-291 aa of NM_001291058.1 Sequence: DCPHKKAVTLLPSFTMTPTATESTASPTTSHRPTTTSHGNVTVHTSSGPTTVTHNPATTTSHGNATISHATVSPTTNGTATSPRSSTVGPHPGPPPPSPSPRSKGALGNYTWANGSQPCVQLQAQIQIRILYPIQGGRKAWGISVLNPNKTKVQGGCDGTHPHLSLSFPYGQLTFGFKQDLHQSPSTVYLDYMAVEYNVSFPQAAQWTFMAQNSSLRELQAPLGQSFCCGNASIVLSPAVHLDLLSLRLQAAQLPDKGHFGPCFSCNRDQS Predict reactive species |
| Full Name | CD68 antigen |
| Calculated Molecular Weight | 35 kDa |
| Observed Molecular Weight | 100-120 kDa |
| GenBank Accession Number | NM_001291058.1 |
| Gene Symbol | Cd68 |
| Gene ID (NCBI) | 12514 |
| RRID | AB_3670757 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purfication |
| UNIPROT ID | P31996-1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for CD68 antibody 83014-5-RR | Download protocol |
| IHC protocol for CD68 antibody 83014-5-RR | Download protocol |
| WB protocol for CD68 antibody 83014-5-RR | Download protocol |
| mouse | IF J Nat Prod Isolation and Biological Activity of Mutgriseorhodins D1 and D2, Two Shunt Products in the Rubromycin Biosynthetic Pathway. Authors - Rui Liu View Article |
| pig | IHC Mol Pharm An (18)F-labeled ECL1i-Based C-C Chemokine Receptor Type 2 Radiotracer for Cardiac Inflammation Imaging. Authors - Shuyang Song View Article |
| rat | IF ACS Appl Mater Interfaces A Fibrous-Porous Microsphere-Based Composite Filler for Synchronized Immediate and Long-Term Soft Tissue Restoration. Authors - Ruixian Lian View Article |
| Zakia (Verified Customer) (08-04-2026) | We tested this CD68 recombinant rabbit monoclonal antibody (Cat No. 83014-5-RR) on mouse brain samples using Western Blot. Overall, the performance was very good. Even though the expected broad smear/band is not clearly visible in our current blot due to our own membrane transfer issues, the signal intensity and specificity look highly promising. We are confident that resolving our optimization and transfer protocol will yield excellent, clean results. It shows great reactivity with mouse tissue, and we highly recommend it for neural and macrophage tracking studies. Applications: Western Blot Primary Antibody Dilution: 1:2000 Cell Tissue Type: mice brain sample CD68 Antibody Western Blot validation (1:2000 dilution) in mice brain sample (Cat no:83014-5-RR) |
| Citations | 16 |
| Dilutions | WB : 1:5000-1:50000 IHC : 1:500-1:2000 IF-P : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 230504G10 |
| Conjugation | Unconjugated |
Mouse CD68 (also known as macrosialin) is a type I transmembrane glycoprotein that is highly and specifically expressed by mouse tissue macrophages, and to a lesser extent by dendritic cells. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages. Due to heavy glycosylation, the apparent molecular mass of CD68 detected by Western blot is generally observed between 80 and 140 kDa, far exceeding its unmodified core protein weight of ~34-37 kDa. Protocols Product Specific Protocols IF protocol for CD68 antibody 83014-5-RR Download protocol IHC protocol for CD68 antibody 83014-5-RR Download protocol WB protocol for CD68 antibody 83014-5-RR Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- MiR-429 influences the development of systemic lupus erythematosus nephritis by targeting SORT1.
Journal: Lupus | Authors: Qiujun Guo | Species: mouse | Application: IHC - Ergothioneine attenuates cardiac fibrosis induced by a high-fat diet in spontaneously hypertensive rats.
Journal: J Nutr Biochem | Authors: Michal Zeman - An (18)F-labeled ECL1i-Based C-C Chemokine Receptor Type 2 Radiotracer for Cardiac Inflammation Imaging.
Journal: Mol Pharm | Authors: Shuyang Song | Species: pig | Application: IHC - Isolation and Biological Activity of Mutgriseorhodins D1 and D2, Two Shunt Products in the Rubromycin Biosynthetic Pathway.
Journal: J Nat Prod | Authors: Rui Liu | Species: mouse | Application: IF - Engineered human plasma-derived cryogels as a multifunctional scaffold to promote diabetic wound healing.
Journal: Mater Today Bio | Authors: Yueming Zhao | Species: rat | Application: IF - A Fibrous-Porous Microsphere-Based Composite Filler for Synchronized Immediate and Long-Term Soft Tissue Restoration.
Journal: ACS Appl Mater Interfaces | Authors: Ruixian Lian | Species: rat | Application: IF - Ginsenoside Rk3 Alleviates Neuroinflammation and Gastrointestinal Dysfunction in Parkinson's Disease via Modulation of Gut Microbiota-Mediated Butyric Acid Metabolism.
Journal: J Agric Food Chem | Authors: Sheng Zhou | Species: mouse | Application: WB,IF - Tracheal delivery of HO-1-modified human umbilical cord-derived mesenchymal stem cells treats pulmonary arterial hypertension by enhancing pulmonary antioxidant and anti-inflammatory responses.
Journal: Transl Res | Authors: Chen Riken - Low-intensity pulsed ultrasound promotes peripheral nerve regeneration by alleviating Schwann cells pyroptosis.
Journal: Commun Biol | Authors: Junfeng Chen - Reduced myocardial NRG-1/ErbB4 signaling is associated with STING-linked macrophage pyroptotic signaling in sepsis-induced cardiac injury.
Journal: Int Immunopharmacol | Authors: Yanian Wei - Clusterin ameliorates diabetic atherosclerosis by suppressing macrophage pyroptosis and activation
Journal: Front Pharmacol | Authors: Lingling Xuan | Species: mouse | Application: IHC - Ergothioneine attenuates cardiac fibrosis induced by a high-fat diet in spontaneously hypertensive rats.
Journal: J Nutr Biochem | Authors: Michal Zeman - An (18)F-labeled ECL1i-Based C-C Chemokine Receptor Type 2 Radiotracer for Cardiac Inflammation Imaging.
Journal: Mol Pharm | Authors: Shuyang Song | Species: pig | Application: IHC - Polystyrene Microplastics Disrupt Vertical Transmission of the Breast Milk Microbiome, Impairing Early-Life Gut Colonization and Immune Development in Offspring.
Journal: FASEB J | Authors: Chenxi Shi | Species: mouse | Application: IHC - Isolation and Biological Activity of Mutgriseorhodins D1 and D2, Two Shunt Products in the Rubromycin Biosynthetic Pathway.
Journal: J Nat Prod | Authors: Rui Liu | Species: mouse | Application: IF - MiR-429 influences the development of systemic lupus erythematosus nephritis by targeting SORT1.
Journal: Lupus | Authors: Qiujun Guo | Species: mouse | Application: IHC
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