IDH3B Monoclonal antibody 68199-1-Ig proteintech
$149.00
In stock
SKU
68199-1-Ig
| Catalog Number | 68199-1-Ig |
| Synonyms | 2D11A9, H IDHB, NAD(+)-specific ICDH subunit beta, RP46 |
| Host | Mouse |
| Reactivity | human, mouse, rat, pig, rabbit |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, ELISA |
| Applications | WB, ELISA |
| Host / Isotype | Mouse / IgG1 |
| Tested Applications — Positive WB detected in | LNCaP cells, Jurkat cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue, pig heart tissue, rabbit heart tissue, rat heart tissue |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Positive WB detected in | LNCaP cells, Jurkat cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue, pig heart tissue, rabbit heart tissue, rat heart tissue |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Tested Reactivity | human, mouse, rat, pig, rabbit |
| Cited Reactivity | human, mouse |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag8693 Product name: Recombinant human IDH3B protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-385 aa of BC001960 Sequence: MAALSGVRWLTRALVSAGNPGAWRGLSTSAAAHAASRSQAEDVRVEGSFPVTMLPGDGVGPELMHAVKEVFKAAAVPVEFQEHHLSEVQNMASEEKLEQVLSSMKENKVAIIGKIHTPMEYKGELASYDMRLRRKLDLFANVVHVKSLPGYMTRHNNLDLVIIREQTEGEYSSLEHESARGVIECLKIVTRAKSQRIAKFAFDYATKKGRGKVTAVHKANIMKLGDGLFLQCCEEVAELYPKIKFETMIIDNCCMQLVQNPYQFDVLVMPNLYGNIIDNLAAGLVGGAGVVPGESYSAEYAVFETGARHPFAQAVGRNIANPTAMLLSASNMLRHLNLEYHSSMIADAVKKVIKVGKVRTRDMGGYSTTTDFIKSVIGHLQTKGS Predict reactive species |
| Full Name | isocitrate dehydrogenase 3 (NAD+) beta |
| Calculated Molecular Weight | 42 kDa |
| Observed Molecular Weight | 39-42 kDa |
| GenBank Accession Number | BC001960 |
| Gene Symbol | IDH3B |
| Gene ID (NCBI) | 3420 |
| RRID | AB_2935288 |
| Conjugate | Unconjugated |
| Purification Method | Protein G purification |
| UNIPROT ID | O43837 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| WB protocol for IDH3B antibody 68199-1-Ig | Download protocol |
| mouse,human | WB Invest Ophthalmol Vis Sci Kaempferol Alleviates Dry Eye Disease Via Modulation of the IDH3B/CCAR2/IKBKB Axis. Authors - Shuyan Zhang View Article |
| mouse | WB,IHC PLoS One Artemether ameliorates type 1 diabetic liver injury alongside the associated defects in mitochondrial ultrastructure and central carbon metabolism. Authors - Qike Fu View Article |
| human | IHC Histochem Cell Biol Fiber type-specific expression of LACTB leverages a function in oxidative metabolism. Authors - Kirsi-Marja Alanen View Article |
| Citations | 3 |
| Dilutions | WB : 1:5000-1:50000 |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Clone Number | 2D11A9 |
| Conjugation | Unconjugated |
Isocitrate dehydrogenase 3 (IDH3) is a key enzyme in the mitochondrial tricarboxylic acid (TCA) cycle, which catalyzes the decarboxylation of isocitrate into α-ketoglutarate and concurrently converts NAD+ into NADH. IDH3B(Isocitrate dehydrogenase 3 (NAD+) beta), also named as RP46, is the beta subunit of IDH3 and controls its substrate levels in the TCA cycle, and it is required for sperm mitochondrial metabolism and spermiogenesis (PMID: 18806796, PMID: 35985423). In addition, DH3B expression increased PFKFB3 protein levels and enhanced glucose uptake, and high expression of IDH3B correlated with poor survival in patients with ESCC, suggesting a potential application of IDH3B in prognosis (PMID: 31053633). Protocols Product Specific Protocols WB protocol for IDH3B antibody 68199-1-Ig Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Kaempferol Alleviates Dry Eye Disease Via Modulation of the IDH3B/CCAR2/IKBKB Axis.
Journal: Invest Ophthalmol Vis Sci | Authors: Shuyan Zhang | Species: mouse,human | Application: WB - Artemether ameliorates type 1 diabetic liver injury alongside the associated defects in mitochondrial ultrastructure and central carbon metabolism.
Journal: PLoS One | Authors: Qike Fu | Species: mouse | Application: WB,IHC - Fiber type-specific expression of LACTB leverages a function in oxidative metabolism.
Journal: Histochem Cell Biol | Authors: Kirsi-Marja Alanen | Species: human | Application: IHC
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