IGF1R/CD221 Recombinant monoclonal antibody 84385-3-RR proteintech
$149.00
In stock
SKU
84385-3-RR
| Catalog Number | 84385-3-RR |
| Synonyms | IGF 1 receptor, IGF1R, IGF-I receptor, IGFIR, IGFR |
| Host | Rabbit |
| Reactivity | human |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | MCF-7 cells, A549 cells, L02 cells |
| Tested Applications — Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | MCF-7 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:10000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Positive WB detected in | MCF-7 cells, A549 cells, L02 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Tested Reactivity | human |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Recombinant Protein Predict reactive species |
| Full Name | IGF I receptor |
| Calculated Molecular Weight | 1367 aa, 155 kDa |
| Observed Molecular Weight | 130 kDa |
| GenBank Accession Number | BC113612 |
| Gene Symbol | IGF1R |
| Gene ID (NCBI) | 3480 |
| RRID | AB_3671921 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purfication |
| UNIPROT ID | P08069 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for IGF1R/CD221 antibody 84385-3-RR | Download protocol |
| IHC protocol for IGF1R/CD221 antibody 84385-3-RR | Download protocol |
| WB protocol for IGF1R/CD221 antibody 84385-3-RR | Download protocol |
| Citations | - |
| Dilutions | WB : 1:2000-1:10000 IHC : 1:500-1:2000 IF/ICC : 1:200-1:800 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 241678B8 |
| Conjugation | Unconjugated |
IGF1R, also named as CD221, is a receptor tyrosine kinase which mediates actions of INS-like growth factor 1 (IGF1). IGF1R binds IGF1 with high affinity and IGF2 and INS with a lower affinity, the activated IGF1R is involved in cell growth and survival control. It is crucial for tumor transformation and survival of malignant cells. IGF1R is translated as ~220 kDa pro-receptors (PMID: 33969359). IGF1R is a heterotetrameric receptor, composed of two alpha subunits (~130 kDa) and two beta subunits (~100-120 kDa) (PMID: 6279656). Both the alpha and beta subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds. Protocols Product Specific Protocols IF protocol for IGF1R/CD221 antibody 84385-3-RR Download protocol IHC protocol for IGF1R/CD221 antibody 84385-3-RR Download protocol WB protocol for IGF1R/CD221 antibody 84385-3-RR Download protocol Standard Protocols Click here to view our Standard Protocols
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