ZEB2 Recombinant monoclonal antibody 82020-1-RR proteintech
$149.00
In stock
SKU
82020-1-RR
| Catalog Number | 82020-1-RR |
| Synonyms | SIP1, 1K22, HRIHFB2411, KIAA0569, SIP 1 |
| Host | Rabbit |
| Reactivity | human |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF-P, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HepG2 cells, HeLa cells, A549 cells, K-562 cells |
| Tested Applications — Positive IHC detected in | human renal cell carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF-P detected in | rat brain tissue |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:10000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:600-1:2400 |
| Recommended Dilutions — Immunofluorescence (IF)-P | IF-P : 1:250-1:1000 |
| Positive WB detected in | HepG2 cells, HeLa cells, A549 cells, K-562 cells |
| Positive IHC detected in | human renal cell carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | rat brain tissue |
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:600-1:2400 |
| Immunofluorescence (IF)-P | IF-P : 1:250-1:1000 |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Ag5171 Product name: Recombinant human ZEB2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-128 aa of BC060819 Sequence: MKQPIMADGPRCKRRKQANPRRKNVVNYDNVVDTGSETDEEDKLHIAEDDGIANPLDQETSPASVPNHESSPHVSQALLPREEEEDEIREGGVEHPWHNNEILQASVDGPGKCLKSLDLFLYHLHIFN Predict reactive species |
| Full Name | zinc finger E-box binding homeobox 2 |
| Calculated Molecular Weight | 136 kDa |
| Observed Molecular Weight | 140-150 kD |
| GenBank Accession Number | BC060819 |
| Gene Symbol | ZEB2 |
| Gene ID (NCBI) | 9839 |
| RRID | AB_2935598 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | O60315 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for ZEB2 antibody 82020-1-RR | Download protocol |
| IHC protocol for ZEB2 antibody 82020-1-RR | Download protocol |
| WB protocol for ZEB2 antibody 82020-1-RR | Download protocol |
| human | WB Noncoding RNA Res Upregulation of LINC02154 promotes esophageal cancer progression by enhancing cell cycling and epithelial-mesenchymal transition Authors - Kotoha Shimote View Article |
| Citations | 1 |
| Dilutions | WB : 1:2000-1:10000 IHC : 1:600-1:2400 IF-P : 1:250-1:1000 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 1K22 |
| Conjugation | Unconjugated |
Zinc finger E-box-binding homeobox (ZEB2) is a key transcription factor that acts as a multifunctional regulator during nervous system development. ZEB2 contains two zinc finger domains and a homeodomain-like sequence and interacts with the TGF-ß superfamily signaling regulators, Smads, to regulate the expression of their downstream genes. ZEB2 is expressed in the developing neural tube, as well as in neural crest cells, the hippocampus and the cerebral cortex. ZEB2 has previously been implicated in EMT, cell-cycle progression, apoptosis and senescence. ZEB2 was overexpressed in bladder, ovarian, stomach, pancreatic and squamous cell carcinoma, in the intestinal subtype of stomach cancers, and at the invasive front of CRC where EMT is most prominen. ZEB2 also mediates cell-fate decision in neuronal, T cells and hematopoietic stem cells. PMID: 27659015 ) Protocols Product Specific Protocols IF protocol for ZEB2 antibody 82020-1-RR Download protocol IHC protocol for ZEB2 antibody 82020-1-RR Download protocol WB protocol for ZEB2 antibody 82020-1-RR Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Upregulation of LINC02154 promotes esophageal cancer progression by enhancing cell cycling and epithelial-mesenchymal transition
Journal: Noncoding RNA Res | Authors: Kotoha Shimote | Species: human | Application: WB
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