ACTA1-Specific Recombinant monoclonal antibody 82714-5-RR proteintech
$149.00
In stock
SKU
82714-5-RR
| Catalog Number | 82714-5-RR |
| Synonyms | ACTA1, Alpha Actin, α actin, 5B15, ACTA |
| Host | Rabbit |
| Reactivity | human, mouse, rat, pig, chicken |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF-P, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | mouse skeletal muscle tissue, rat skeletal muscle tissue, pig skeletal muscle tissue, chicken skeletal muscle tissue |
| Tested Applications — Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF-P detected in | mouse skeletal muscle tissue |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Recommended Dilutions — Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Positive WB detected in | mouse skeletal muscle tissue, rat skeletal muscle tissue, pig skeletal muscle tissue, chicken skeletal muscle tissue |
| Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse skeletal muscle tissue |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat, pig, chicken |
| Cited Reactivity | human, mouse |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Peptide Predict reactive species |
| Full Name | actin, alpha 1, skeletal muscle |
| Calculated Molecular Weight | 42 kDa |
| Observed Molecular Weight | 39-42 kDa |
| GenBank Accession Number | NM_001100 |
| Gene Symbol | ACTA1 |
| Gene ID (NCBI) | 58 |
| RRID | AB_3086515 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | P68133 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for ACTA1-Specific antibody 82714-5-RR | Download protocol |
| IHC protocol for ACTA1-Specific antibody 82714-5-RR | Download protocol |
| WB protocol for ACTA1-Specific antibody 82714-5-RR | Download protocol |
| human,mouse | WB J Adv Res OTUD7B attenuates renal fibrosis by regulating PRDX1 protein stability. Authors - Yufeng Xiong KD Validated View Article |
| Citations | 1 |
| Dilutions | WB : 1:5000-1:50000 IHC : 1:500-1:2000 IF-P : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 5B15 |
| Conjugation | Unconjugated |
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTA1 gene encodes skeletal muscle alpha-actin, the principal actin isoform in adult skeletal muscle, which interacts with a variety of proteins to produce the force for muscle contraction. There is only the N-termninal 8 amino acids difference among the actin family members. This antibody is specific to the ACTA1. It does not cross-react with other actin isoforms. Protocols Product Specific Protocols IF protocol for ACTA1-Specific antibody 82714-5-RR Download protocol IHC protocol for ACTA1-Specific antibody 82714-5-RR Download protocol WB protocol for ACTA1-Specific antibody 82714-5-RR Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- OTUD7B attenuates renal fibrosis by regulating PRDX1 protein stability.
Journal: J Adv Res | Authors: Yufeng Xiong KD Validated | Species: human,mouse | Application: WB
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