ARID1A Recombinant monoclonal antibody 83733-3-RR proteintech
$149.00
In stock
SKU
83733-3-RR
| Catalog Number | 83733-3-RR |
| Synonyms | BAF250A, BAF250, B120, AT-rich interactive domain-containing protein 1A, ARID domain-containing protein 1A |
| Host | Rabbit |
| Reactivity | human |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | MCF-7 cells, BxPC-3 cells, K-562 cells, HepG2 cells |
| Tested Applications — Positive IHC detected in | human colon cancer tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HeLa cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:150-1:600 |
| Positive WB detected in | MCF-7 cells, BxPC-3 cells, K-562 cells, HepG2 cells |
| Positive IHC detected in | human colon cancer tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:150-1:600 |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Ag30749 Product name: Recombinant human ARID1A protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1230-1370 aa of NM_006015 Sequence: KDPYGSMRKAPGSDPFMSSGQGPNGGMGDPYSRAAGPGLGNVAMGPRQHYPYGGPYDRVRTEPGIGPEGNMSTGAPQPNLMPSNPDSGMYSPSRYPPQQQQQQQQRHDSYGNQFSTQGTPSGSPFPSQQTTMYQQQQQNYK Predict reactive species |
| Full Name | AT rich interactive domain 1A (SWI-like) |
| Calculated Molecular Weight | 242 kDa |
| Observed Molecular Weight | 250-260 kDa |
| GenBank Accession Number | NM_006015 |
| Gene Symbol | ARID1A |
| Gene ID (NCBI) | 8289 |
| RRID | AB_3671333 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | O14497 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for ARID1A antibody 83733-3-RR | Download protocol |
| IHC protocol for ARID1A antibody 83733-3-RR | Download protocol |
| WB protocol for ARID1A antibody 83733-3-RR | Download protocol |
| human | WB Nat Commun Counteracting FOX proteins epigenetically control the herpesvirus lytic-latent balance. Authors - Yuhang Xiang View Article |
| Citations | 1 |
| Dilutions | WB : 1:5000-1:50000 IHC : 1:50-1:500 IF/ICC : 1:150-1:600 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 240690F1 |
| Conjugation | Unconjugated |
ARID1A, also named as BAF250, BAF250A, C1orf4, OSA1 and SMARCF1, is involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). It binds DNA non-specifically. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. ARID1A belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The antibody is specific to ARID1A. Protocols Product Specific Protocols IF protocol for ARID1A antibody 83733-3-RR Download protocol IHC protocol for ARID1A antibody 83733-3-RR Download protocol WB protocol for ARID1A antibody 83733-3-RR Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Counteracting FOX proteins epigenetically control the herpesvirus lytic-latent balance.
Journal: Nat Commun | Authors: Yuhang Xiang | Species: human | Application: WB
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