CCDC94 Recombinant monoclonal antibody 83580-1-RR proteintech
$149.00
In stock
SKU
83580-1-RR
| Catalog Number | 83580-1-RR |
| Synonyms | 240517A1, Coiled-coil domain-containing protein 94, YJU2 |
| Host | Rabbit |
| Reactivity | human |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IF/ICC, FC (Intra), ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HeLa cells, HEK-293 cells, Jurkat cell, K-562 cells, HepG2 cells |
| Tested Applications — Positive IF/ICC detected in | A549 cells, A431 cells |
| Tested Applications — Positive FC (Intra) detected in | A431 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:10000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Positive WB detected in | HeLa cells, HEK-293 cells, Jurkat cell, K-562 cells, HepG2 cells |
| Positive IF/ICC detected in | A549 cells, A431 cells |
| Positive FC (Intra) detected in | A431 cells |
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Ag13764 Product name: Recombinant human CCDC94 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-323 aa of BC000561 Sequence: MSERKVLNKYYPPDFDPSKIPKLKLPKDRQYVVRLMAPFNMRCKTCGEYIYKGKKFNARKETVQNEVYLGLPIFRFYIKCTRCLAEITFKTDPENTDYTMEHGATRNFQAEKLLEEEEKRVQKEREDEELNNPMKVLENRTKDSKLEMEVLENLQELKDLNQRQAHVDFEAMLRQHRLSEEERRRQQQEEDEQETAALLEEARKRRLLEDSDSEDEAAPSPLQPALRPNPTAILDEAPKPKRKVEVWEQSVGSLGSRPPLSRLVVVKKAKADPDCSNGQPQAAPTPGAPQNRKEANPTPLTPGASSLSQLGAYLDSDDSNGSN Predict reactive species |
| Full Name | coiled-coil domain containing 94 |
| Calculated Molecular Weight | 323 aa, 37 kDa |
| Observed Molecular Weight | 40 kDa |
| GenBank Accession Number | BC000561 |
| Gene Symbol | CCDC94 |
| Gene ID (NCBI) | 55702 |
| RRID | AB_3671193 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9BW85 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| FC protocol for CCDC94 antibody 83580-1-RR | Download protocol |
| IF protocol for CCDC94 antibody 83580-1-RR | Download protocol |
| WB protocol for CCDC94 antibody 83580-1-RR | Download protocol |
| Citations | - |
| Dilutions | WB : 1:2000-1:10000 IF/ICC : 1:125-1:500 FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 240517A1 |
| Conjugation | Unconjugated |
CCDC94, also known as YJU2, is a splicing factor, predicted to be involved in RNA splicing and negative regulation of DNA damage response, signal transduction by p53 class mediator. Part of U2-type catalytic step 1 spliceosome. And that it is required for pre-mRNA splicing both in vivo and in vitro. Yju2 acts in concert with an unidentified heat-resistant factor(s) in an ATP-independent manner to promote the first catalytic reaction of pre-mRNA splicing after Prp2-mediated structural rearrangement of the spliceosome. (PMID: 17515604) Protocols Product Specific Protocols FC protocol for CCDC94 antibody 83580-1-RR Download protocol IF protocol for CCDC94 antibody 83580-1-RR Download protocol WB protocol for CCDC94 antibody 83580-1-RR Download protocol Standard Protocols Click here to view our Standard Protocols
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