CDCA7L Recombinant monoclonal antibody 83682-4-RR proteintech
$149.00
In stock
SKU
83682-4-RR
| Catalog Number | 83682-4-RR |
| Synonyms | 240639A3, Cell division cycle-associated 7-like protein, HR1, JPO2, Protein JPO2 |
| Host | Rabbit |
| Reactivity | human |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, FC (Intra), ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | A549 cells, HEK-293T cells, HeLa cells, K-562 cells, MCF-7 cells |
| Tested Applications — Positive IHC detected in | human rectal cancer tissue, human placenta tissue, human appendicitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive FC (Intra) detected in | A431 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:2000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Positive WB detected in | A549 cells, HEK-293T cells, HeLa cells, K-562 cells, MCF-7 cells |
| Positive IHC detected in | human rectal cancer tissue, human placenta tissue, human appendicitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | A431 cells |
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Ag10944 Product name: Recombinant human CDCA7L protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-269 aa of BC009352 Sequence: MELATRYQIPKEVADIFNAPSDDEEFVGFRDDVPMETLSSEESCDSFDSLESGKQQDVRFHSKYFTEELRRIFIEDTDSETEDFAGFTQSDLNGKTNPEVMVVESDLSDDGKASLVSEEEEDEEEDKATPRRSRSRRSSIGLRVAFQFPTKKLANKPDKNSSSEQLFSSARLQNEKKTILERKKDCRQVIQREDSTSESEDDSRDESQESSDALLKRTMNIKENKAMLAQLLAELNSMPDFFPVRTPTSASRKKTVRRAFSEGQITRRM Predict reactive species |
| Full Name | cell division cycle associated 7-like |
| Calculated Molecular Weight | 454 aa, 52 kDa |
| Observed Molecular Weight | 52 kDa |
| GenBank Accession Number | BC009352 |
| Gene Symbol | CDCA7L |
| Gene ID (NCBI) | 55536 |
| RRID | AB_3671283 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q96GN5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| FC protocol for CDCA7L antibody 83682-4-RR | Download protocol |
| WB protocol for CDCA7L antibody 83682-4-RR | Download protocol |
| Citations | - |
| Dilutions | WB : 1:500-1:2000 IHC : 1:1000-1:4000 FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 240639A3 |
| Conjugation | Unconjugated |
Cell division cycle-associated 7-like protein (CDCA7L) is also known as HR1 and JPO2 and belongs to the JPO protein family. CDCA7L is identified as a target gene of c-Myc and is frequently dysregulated in multiple cancers. CDCA7L can complement the c-Myc transformation-defective mutant W135E and potentiate Myc-mediated transformation (PMID: 24141559, PMID: 37140023). CDCA7L translocates from cytoplasm to nucleus under dexamethasone induction. CDCA7L is widely expressed. CDCA7L has 4 isoforms produced by alternative splicing (refer to UniProt). Protocols Product Specific Protocols FC protocol for CDCA7L antibody 83682-4-RR Download protocol WB protocol for CDCA7L antibody 83682-4-RR Download protocol Standard Protocols Click here to view our Standard Protocols
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