CEACAM1/3/6 (CD66a/c/d) Recombinant monoclonal antibody 83524-3-RR proteintech
$149.00
In stock
SKU
83524-3-RR
| Catalog Number | 83524-3-RR |
| Synonyms | CEACAM1, CEACAM1/CD66a, 240497B4, BGP, BGP1 |
| Host | Rabbit |
| Reactivity | human |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HepG2 cells, HT-29 cells |
| Tested Applications — Positive IHC detected in | human ovary cancer tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HT-29 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:10000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Positive WB detected in | HepG2 cells, HT-29 cells |
| Positive IHC detected in | human ovary cancer tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HT-29 cells |
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Tested Reactivity | human |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Eg0314 Product name: Recombinant Human CEACAM-1/CD66a protein (His Tag)(HPLC verified) Source: mammalian cells -derived, pHZ-KIsec-C-6*HIS Tag: C-10*HIS Domain: 35-428 aa of BC014473 Sequence: QLTTESMPFNVAEGKEVLLLVHNLPQQLFGYSWYKGERVDGNRQIVGYAIGTQQATPGPANSGRETIYPNASLLIQNVTQNDTGFYTLQVIKSDLVNEEATGQFHVYPELPKPSISSNNSNPVEDKDAVAFTCEPETQDTTYLWWINNQSLPVSPRLQLSNGNRTLTLLSVTRNDTGPYECEIQNPVSANRSDPVTLNVTYGPDTPTISPSDTYYRPGANLSLSCYAASNPPAQYSWLINGTFQQSTQELFIPNITVNNSGSYTCHANNSVTGCNRTTVKTIIVTELSPVVAKPQIKASKTTVTGDKDSVNLTCSTNDTGISIRWFFKNQSLPSSERMKLSQGNTTLSINPVKREDAGTYWCEVFNPISKNQSDPIMLNVNYNALPQENGLSPG Predict reactive species |
| Full Name | carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein) |
| Calculated Molecular Weight | 526 aa, 58 kDa |
| Observed Molecular Weight | 135-150 kDa |
| GenBank Accession Number | BC014473 |
| Gene Symbol | CEACAM1 |
| Gene ID (NCBI) | 634 |
| RRID | AB_3671151 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | P13688 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for CEACAM1/3/6 (CD66a/c/d) antibody 83524-3-RR | Download protocol |
| IHC protocol for CEACAM1/3/6 (CD66a/c/d) antibody 83524-3-RR | Download protocol |
| WB protocol for CEACAM1/3/6 (CD66a/c/d) antibody 83524-3-RR | Download protocol |
| Citations | - |
| Dilutions | WB : 1:2000-1:10000 IHC : 1:300-1:1200 IF/ICC : 1:200-1:800 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 240497B4 |
| Conjugation | Unconjugated |
CEACAM1, also known as CD66a or BGP (biliary glycoprotein), is a member of the cacinoembryonic antigen (CEA) family which belongs to the immunoglobulin superfamily of cell adhesion molecules (PMID: 16919437). CEACAMs play major roles in cell-cell and cell-ECM adhesion and have been implicated in the control of cell proliferation, angiogenesis, and tissue remodeling (PMID: 23021083). CEACAM1 is a transmembrane glycoprotein that is expressed on epithelial, endothelial, and immune cells (PMID: 30314283). CEACAM1 has been demonstrated to play a role in morphogenesis, apoptosis, angiogenesis, cell proliferation, cell motility, fibrosis, and immunity (PMID: 31604530). Protocols Product Specific Protocols IF protocol for CEACAM1/3/6 (CD66a/c/d) antibody 83524-3-RR Download protocol IHC protocol for CEACAM1/3/6 (CD66a/c/d) antibody 83524-3-RR Download protocol WB protocol for CEACAM1/3/6 (CD66a/c/d) antibody 83524-3-RR Download protocol Standard Protocols Click here to view our Standard Protocols
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