IL-12A/IL-12 p35 Recombinant monoclonal antibody 85754-4-RR proteintech
$149.00
In stock
SKU
85754-4-RR
| Catalog Number | 85754-4-RR |
| Synonyms | IL-12, CLMF, CLMF p35, IL 12 subunit p35, IL 12A |
| Host | Rabbit |
| Reactivity | human |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | Recombinant protein |
| Tested Applications — Positive IF/ICC detected in | IFN gamma and LPS treated THP-1 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| Positive WB detected in | Recombinant protein |
| Positive IF/ICC detected in | IFN gamma and LPS treated THP-1 cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Eg3341 Product name: Recombinant Human IL12A protein (rFc Tag) Source: mammalian cells -derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 23-219 aa of BC104982 Sequence: RNLPVATPDPGMFPCLHHSQNLLRAVSNMLQKARQTLEFYPCTSEEIDHEDITKDKTSTVEACLPLELTKNESCLNSRETSFITNGSCLASRKTSFMMALCLSSIYEDLKMYQVEFKTMNAKLLMDPKRQIFLDQNMLAVIDELMQALNFNSETVPQKSSLEEPDFYKTKIKLCILLHAFRIRAVTIDRVMSYLNAS Predict reactive species |
| Full Name | interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35) |
| Calculated Molecular Weight | 25 kDa |
| Observed Molecular Weight | 65 kDa |
| GenBank Accession Number | BC104982 |
| Gene Symbol | IL-12A |
| Gene ID (NCBI) | 3592 |
| RRID | AB_3744349 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | P29459 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for IL-12A/IL-12 p35 antibody 85754-4-RR | Download protocol |
| WB protocol for IL-12A/IL-12 p35 antibody 85754-4-RR | Download protocol |
| human | IF Life Sci Interleukin-35 alleviates pulmonary arterial hypertension by suppressing the VEGFA/VEGFR2 signaling pathway. Authors - Jie Feng View Article |
| Citations | 1 |
| Dilutions | WB : 1:5000-1:50000 IF/ICC : 1:250-1:1000 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 242887G5 |
| Conjugation | Unconjugated |
IL-12A (Interleukin-12A) is a subunit of the cytokine IL-12, which is also known as IL-12 p35. IL-12 is a heterodimeric cytokine composed of two subunits: IL-12A (p35) and IL-12B (p40). This cytokine plays a crucial role in the immune response, particularly in the activation and differentiation of T cells and natural killer (NK) cells. This antibody recognizes IL-12A and IL-12 P70, which consists of IL-12A and B, but does not recognize IL-12B. Protocols Product Specific Protocols IF protocol for IL-12A/IL-12 p35 antibody 85754-4-RR Download protocol WB protocol for IL-12A/IL-12 p35 antibody 85754-4-RR Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Interleukin-35 alleviates pulmonary arterial hypertension by suppressing the VEGFA/VEGFR2 signaling pathway.
Journal: Life Sci | Authors: Jie Feng | Species: human | Application: IF
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