GCLM Recombinant monoclonal antibody 82623-1-RR proteintech
$149.00
In stock
SKU
82623-1-RR
| Catalog Number | 82623-1-RR |
| Synonyms | Gamma-ECS regulatory subunit, Gamma-glutamylcysteine synthetase regulatory subunit, GLCLR, Glutamate--cysteine ligase modifier subunit, Glutamate--cysteine ligase regulatory subunit |
| Host | Rabbit |
| Reactivity | human, mouse, rat and More (1) |
| Reactivity | human, mouse, rat, monkey |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HeLa cells, RAW 264.7 cells, HEK-293 cells, A431 cells, Jurkat cells, K-562 cells, HSC-T6 cells, mouse liver tissue, rat liver tissue |
| Tested Applications — Positive IHC detected in | mouse brain tissue, human ovarian cancer, human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Positive WB detected in | HeLa cells, RAW 264.7 cells, HEK-293 cells, A431 cells, Jurkat cells, K-562 cells, HSC-T6 cells, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | mouse brain tissue, human ovarian cancer, human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | monkey |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Ag5497 Product name: Recombinant human GCLM protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-274 aa of BC041809 Sequence: MGTDSRAAKALLARARTLHLQTGNLLNWGRLRKKCPSTHSEELHDCIQKTLNEWSSQINPDLVREFPDVLECTVSHAVEKINPDEREEMKVSAKLFIVESNSSSSTRSAVDMACSVLGVAQLDSVIIASPPIEDGVNLSLEHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPKELLSEASFQEALQESIPDIQAHEWVPLWLLRYSVIVKSRGIIKSKGYILQAKRRGS Predict reactive species |
| Full Name | glutamate-cysteine ligase, modifier subunit |
| Calculated Molecular Weight | 31 kDa |
| Observed Molecular Weight | 31 kDa |
| GenBank Accession Number | BC041809 |
| Gene Symbol | GCLM |
| Gene ID (NCBI) | 2730 |
| RRID | AB_3086493 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | P48507 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for GCLM antibody 82623-1-RR | Download protocol |
| WB protocol for GCLM antibody 82623-1-RR | Download protocol |
| monkey | WB Microorganisms Biochanin A Exerts Broad-Spectrum Antiviral Activity Against Coronaviruses via Activating the AMPK/Nrf2/GSH Pathway. Authors - Qisheng Lin View Article |
| Citations | 1 |
| Dilutions | WB : 1:5000-1:50000 IHC : 1:500-1:2000 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 4K16 |
| Conjugation | Unconjugated |
GCLM(Glutamate--cysteine ligase regulatory subunit) is also named as GLCLR and belongs to the aldo/keto reductase family. It catalyzes the first step of GSH synthesis and is involved in gamma-glutamyl cycle. GCLM is implicated in some forms of hemolytic anemia. Protocols Product Specific Protocols IHC protocol for GCLM antibody 82623-1-RR Download protocol WB protocol for GCLM antibody 82623-1-RR Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Biochanin A Exerts Broad-Spectrum Antiviral Activity Against Coronaviruses via Activating the AMPK/Nrf2/GSH Pathway.
Journal: Microorganisms | Authors: Qisheng Lin | Species: monkey | Application: WB
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