GFAP Recombinant monoclonal antibody 81063-1-RR proteintech
$149.00
In stock
SKU
81063-1-RR
| Catalog Number | 81063-1-RR |
| Synonyms | glial fibrillary acidic protein |
| Host | Rabbit |
| Reactivity | human, mouse, rat, pig |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IHC-Autostainer, IF/ICC, IF-P, IF-Fro, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | U-251 cells, rat brain tissue, mouse brain tissue, pig brain tissue |
| Tested Applications — Positive IHC-Autostainer detected in | human brain tissue |
| Tested Applications — Positive IHC detected in | rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF-P detected in | rat brain tissue, mouse brain tissue |
| Tested Applications — Positive IF-Fro detected in | mouse brain tissue, rat brain tissue |
| Tested Applications — Positive IF/ICC detected in | U-251 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:4950-1:19800 |
| Recommended Dilutions — Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Positive WB detected in | U-251 cells, rat brain tissue, mouse brain tissue, pig brain tissue |
| Positive IHC-Autostainer detected in | human brain tissue |
| Positive IHC detected in | rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | rat brain tissue, mouse brain tissue |
| Positive IF-Fro detected in | mouse brain tissue, rat brain tissue |
| Positive IF/ICC detected in | U-251 cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC)-AUTOSTAINER | IHC-AUTOSTAINER : 1:5000-1:20000 |
| Immunohistochemistry (IHC) | IHC : 1:4950-1:19800 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Tested Reactivity | human, mouse, rat, pig |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Ag10423 Product name: Recombinant human GFAP protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 83-432 aa of BC013596 Sequence: YIEKVRFLEQQNKALAAELNQLRAKEPTKLADVYQAELRELRLRLDQLTANSARLEVERDNLAQDLATVRQKLQDETNLRLEAENNLAAYRQEADEATLARLDLERKIESLEEEIRFLRKIHEEEVRELQEQLARQQVHVELDVAKPDLTAALKEIRTQYEAMASSNMHEAEEWYRSKFADLTDAAARNAELLRQAKHEANDYRRQLQSLTCDLESLRGTNESLERQMREQEERHVREAASYQEALARLEEEGQSLKDEMARHLQEYQDLLNVKLALDIEIATYRKLLEGEENRITIPVQTFSNLQIRETSLDTKSVSEGHLKRNIVVKTVEMRDGEVIKESKQEHKDVM Predict reactive species |
| Full Name | glial fibrillary acidic protein |
| Calculated Molecular Weight | 432 aa, 50 kDa |
| Observed Molecular Weight | 45-50 kDa |
| GenBank Accession Number | BC013596 |
| Gene Symbol | GFAP |
| Gene ID (NCBI) | 2670 |
| RRID | AB_2923699 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | P14136 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for GFAP antibody 81063-1-RR | Download protocol |
| IHC protocol for GFAP antibody 81063-1-RR | Download protocol |
| WB protocol for GFAP antibody 81063-1-RR | Download protocol |
| Kateryna (Verified Customer) (01-23-2025) | We use the GFAP antibody to visualize astrocytes via immunostaining in mouse brain tissue. The mouse brain tissue is fixed, brain sections are 40 μm thick, and blocked with 3% normal goat serum. The incubation with the primary antibody (GFAP) is performed at 4°C overnight, followed by the corresponding Alexa 633-labeled anti-rabbit fluorescent secondary antibody. Applications: Immunofluorescence Primary Antibody Dilution: 1:100 Cell Tissue Type: Mouse brain tissue GFAP Antibody Immunofluorescence validation (1:100 dilution) in Mouse brain tissue (Cat no:81063-1-RR) |
| Citations | 1 |
| Dilutions | WB : 1:5000-1:50000 IHC-AUTOSTAINER : 1:5000-1:20000 IHC : 1:4950-1:19800 IF-P : 1:50-1:500 IF-FRO : 1:50-1:500 IF/ICC : 1:200-1:800 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 4C6 |
| Conjugation | Unconjugated |
GFAP (Glial fibrillary acidic protein ), an intermediate-filament (IF) protein , is specifically expressed in cells of astroglial lineage and is widely used to mark astroglia in the brain. It is also used as a marker for intracranial and intraspinal tumors arising from astrocytes. Protocols Product Specific Protocols IF protocol for GFAP antibody 81063-1-RR Download protocol IHC protocol for GFAP antibody 81063-1-RR Download protocol WB protocol for GFAP antibody 81063-1-RR Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Edaravone-dexborneol improves neurological function after cerebral ischemia-reperfusion injury by activating NRF2/p62 and suppressing NF-κB/NLRP3 signaling.
Journal: Eur J Pharmacol | Authors: Zezhou Xu
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