IREB2 Polyclonal antibody 29976-1-AP proteintech
$149.00
In stock
SKU
29976-1-AP
| Catalog Number | 29976-1-AP |
| Synonyms | IRE BP 2, IREB 2, IRE-BP 2, IREBP2, Iron-responsive element-binding protein 2 |
| Host | Rabbit |
| Reactivity | human, mouse and More (1) |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | mouse liver tissue |
| Tested Applications — Positive IP detected in | A549 cells, mouse liver tissue |
| Tested Applications — Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | A549 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:8000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Positive WB detected in | mouse liver tissue |
| Positive IP detected in | A549 cells, mouse liver tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag32233 Product name: Recombinant human IREB2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 50-255 aa of BC017880 Sequence: VRNCDGFLMKKEDVMNILDWKTKQSNVEVPFFPARVLLQDFTGIPAMVDFAAMREAVKTLGGDPEKVHPACPTDLTVDHSLQIDFSKCAIQNAPNPGGGDLQKAGKLSPLKVQPKKLPCRGQTTCRGSCDSGELGRNSGTFSSQIENTPILCPFHLQPVPEPETVLKNQEVEFGRNRERLQFFKWSSRVFKNVAVIPPGTGMAHQI Predict reactive species |
| Full Name | iron-responsive element binding protein 2 |
| Calculated Molecular Weight | 105 kDa |
| Observed Molecular Weight | 100-105 kDa |
| GenBank Accession Number | BC017880 |
| Gene Symbol | IREB2 |
| Gene ID (NCBI) | 3658 |
| RRID | AB_3086201 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P48200 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for IREB2 antibody 29976-1-AP | Download protocol |
| IHC protocol for IREB2 antibody 29976-1-AP | Download protocol |
| IP protocol for IREB2 antibody 29976-1-AP | Download protocol |
| WB protocol for IREB2 antibody 29976-1-AP | Download protocol |
| mouse,human | WB Nat Cell Biol Peritumoural adipose tissue promotes ferroptosis resistance by 3-hydroxykynurenine-mediated suppression of ferritinophagy. Authors - Yan-Yu Zhang View Article |
| mouse | WB Front Cell Dev Biol The D826V point mutation in IREB2 induces lipogenesis in adipose tissues. Authors - Yibing Lv View Article |
| Citations | 11 |
| Dilutions | WB : 1:1000-1:8000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:200-1:800 IF/ICC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
IREB2 is an RNA-binding protein that acts to regulate iron levels in the cells by regulating the translation and stability of mRNAs that affect iron homeostasis under conditions when iron is depleted. When iron levels are low, this protein binds to iron-responsive elements (IRES), stem-loop structures located either in the 5' or 3' UTRs. Binding to the 5' UTR represses translation, while binding to the 3' UTR inhibits mRNA degradation. When iron is found in the cell, this protein is degraded in a F-box and leucine rich repeat protein 5-dependent manner. Protocols Product Specific Protocols IF protocol for IREB2 antibody 29976-1-AP Download protocol IHC protocol for IREB2 antibody 29976-1-AP Download protocol IP protocol for IREB2 antibody 29976-1-AP Download protocol WB protocol for IREB2 antibody 29976-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- LRRK2 mutations block NCOA4 trafficking upon iron overload leading to ferroptotic death.
Journal: J Cell Sci | Authors: Andres Goldman - Targeting NFS1-mediated ferroptosis mitigates alveolar injury in experimental idiopathic pulmonary fibrosis.
Journal: Toxicol Appl Pharmacol | Authors: Jianzhong Li - Peritumoural adipose tissue promotes ferroptosis resistance by 3-hydroxykynurenine-mediated suppression of ferritinophagy.
Journal: Nat Cell Biol | Authors: Yan-Yu Zhang | Species: mouse,human | Application: WB - HERC2 as a bidirectional iron metabolism switch: Synergistic NCOA4 stabilization and FBXL5 degradation drive ferroptosis-cascaded hepatotoxicity in Nd(2)O(3) nanoparticles exposure.
Journal: Free Radic Biol Med | Authors: Sihao Liu - The D826V point mutation in IREB2 induces lipogenesis in adipose tissues.
Journal: Front Cell Dev Biol | Authors: Yibing Lv | Species: mouse | Application: WB - Liraglutide and GLP-1(9-37) alleviated hepatic ischemia-reperfusion injury by inhibiting ferroptosis via GSK3β/Nrf2 pathway and SMAD159/Hepcidin/FTH pathway
Journal: Redox Biol | Authors: Chenqi Lu | Application: WB
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