IRG1 Polyclonal antibody 28436-1-AP proteintech
$149.00
In stock
SKU
28436-1-AP
| Catalog Number | 28436-1-AP |
| Synonyms | ACOD1, CAD |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | IFN gamma+LPS treated THP-1 cells, LPS treated RAW 264.7 cells |
| Tested Applications — Positive IF/ICC detected in | LPS treated RAW 264.7 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:2000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Positive WB detected in | IFN gamma+LPS treated THP-1 cells, LPS treated RAW 264.7 cells |
| Positive IF/ICC detected in | LPS treated RAW 264.7 cells |
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag28913 Product name: Recombinant human IRG1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 285-481 aa of NM_001258406 Sequence: DAAASVRKHLVAERALLPTDYIKRIVLRIPNVQYVNRPFPVSEHEARHSFQYVACAMLLDGGITVPSFHECQINRPQVRELLSKVELEYPPDNLPSFNILYCEISVTLKDGATFTDRSDTFYGHWRKPLSQEDLEEKFRANASKMLSWDTVESLIKIVKNLEDLEDCSVLTTLLKGPSPPEVASNSPACNNSITNLS Predict reactive species |
| Full Name | immunoresponsive 1 homolog (mouse) |
| Calculated Molecular Weight | 53 kDa |
| Observed Molecular Weight | 53 kDa |
| GenBank Accession Number | NM_001258406 |
| Gene Symbol | IRG1 |
| Gene ID (NCBI) | 730249 |
| RRID | AB_2935468 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | A6NK06 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for IRG1 antibody 28436-1-AP | Download protocol |
| WB protocol for IRG1 antibody 28436-1-AP | Download protocol |
| mouse | WB Apoptosis IRG1/Itaconate inhibits proliferation and promotes apoptosis of CD69+CD103+CD8+ tissue-resident memory T cells in autoimmune hepatitis by regulating the JAK3/STAT3/P53 signalling pathway Authors - Pei Zhou View Article |
| human | WB,IF Apoptosis Itaconate promotes mitophagy to inhibit neuronal ferroptosis after subarachnoid hemorrhage Authors - Guijun Wang View Article |
| Lisa (Verified Customer) (08-14-2025) | Works perfectly for HEK cells; even highly diluted Applications: Western Blot Primary Antibody Dilution: 1:2000 |
| Citations | 12 |
| Dilutions | WB : 1:500-1:2000 IF/ICC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
The host Immune-Responsive Gene 1 (IRG1; also called Acod1) is a mitochondrial enzyme induced under inflammatory conditions that produces the metabolite itaconate by decarboxylating cis-aconitate, a TCA cycle intermediate. Gene expression profiling studies of murine macrophages and microglial cells have revealed that IRG1 is highly expressed under pro-inflammatory conditions. Furthermore, IRG1 is highly expressed in the pregnant uterus during the early events leading to implantation, the specific phase of pregnancy in which high levels of inflammatory cytokines are secreted. IRG1 localizes to the mitochondria and may represent a key link between immunological and metabolic processes. IRG1 has crucial functions in embryonic implantation and neurodegeneration. Also, IRG1 promotes endotoxin tolerance by increasing A20 expression in macrophages via increased ROS production. (PMID: 25640654) Protocols Product Specific Protocols IF protocol for IRG1 antibody 28436-1-AP Download protocol WB protocol for IRG1 antibody 28436-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Harnessing Kupffer Cell Metabolic Rewiring: Rapamycin-Gliadin Nanoparticle as a Pivotal Strategy for Immune Tolerance in Celiac Disease
Journal: ACS Nano | Authors: Xiaohan Jiang KD Validated | Species: mouse | Application: WB - Acid neutralization and metal-phenolic nanomedicine on-demand release microspheres for reversing cartilage degeneration.
Journal: J Control Release | Authors: Sixiang Wang KD Validated | Species: mouse | Application: WB,IF - IRG1/itaconate enhances efferocytosis by activating Nrf2-TIM4 signaling pathway to alleviate con A induced autoimmune liver injury
Journal: Cell Commun Signal | Authors: Liwu Zeng KO Validated | Species: mouse | Application: WB - Activity-based chemical proteomics reveals caffeic acid ameliorates pentylenetetrazol-induced seizures by covalently targeting aconitate decarboxylase 1
Journal: Cell Commun Signal | Authors: Guanjun Li | Species: mouse | Application: WB - IRG1/Itaconate inhibits proliferation and promotes apoptosis of CD69+CD103+CD8+ tissue-resident memory T cells in autoimmune hepatitis by regulating the JAK3/STAT3/P53 signalling pathway
Journal: Apoptosis | Authors: Pei Zhou | Species: mouse | Application: WB - Itaconate promotes mitophagy to inhibit neuronal ferroptosis after subarachnoid hemorrhage
Journal: Apoptosis | Authors: Guijun Wang | Species: human | Application: WB,IF
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