MDC1 Recombinant monoclonal antibody 83496-1-RR proteintech
$149.00
In stock
SKU
83496-1-RR
| Catalog Number | 83496-1-RR |
| Synonyms | NFBD1, NFBD 1, MDC 1, 240469D5 |
| Host | Rabbit |
| Reactivity | human |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | human testis tissue, HeLa cells, HEK-293 cells, MCF-7 cells, K-562 cells, PC-3 cells |
| Tested Applications — Positive IF/ICC detected in | HeLa cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:2000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:150-1:600 |
| Positive WB detected in | human testis tissue, HeLa cells, HEK-293 cells, MCF-7 cells, K-562 cells, PC-3 cells |
| Positive IF/ICC detected in | HeLa cells |
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:150-1:600 |
| Tested Reactivity | human |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Ag20135 Product name: Recombinant human MDC1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC152556 Sequence: MEDTQAIDWDVEEEEETEQSSESLRCNVEPVGRLHIFSGAHGPEKDFPLHLGKNVVGRMPDCSVALPFPSISKQHAEIEILAWDKAPILRDCGSLNGTQILRPPKVLSPGVSHRLRDQELILFADLLCQYHRLDVSLPFVSRGPLTVEETPRVQGETQPQRLLLAEDSEEEVDFLSERRMVKKSRTTSSSVIVPESDEEGHSPVLGGLGPPFAFNLNSDTDVEEGQQPATEEASSAARRGATVEAKQSEAEVVTEIQLEKDQPLVKERDNDTKVKRGAGNGVVPAGVILERSQPPGEDSDTDVDDDSRPPGRPAEVHLERAQPFGFIDSDTDAEEERIPATPVVIPMKKR Predict reactive species |
| Full Name | mediator of DNA damage checkpoint 1 |
| Calculated Molecular Weight | 2089 aa, 227 kDa |
| Observed Molecular Weight | 260-320 kDa |
| GenBank Accession Number | BC152556 |
| Gene Symbol | MDC1 |
| Gene ID (NCBI) | 9656 |
| RRID | AB_3671123 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | Q14676 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for MDC1 antibody 83496-1-RR | Download protocol |
| WB protocol for MDC1 antibody 83496-1-RR | Download protocol |
| Citations | - |
| Dilutions | WB : 1:500-1:2000 IF/ICC : 1:150-1:600 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 240469D5 |
| Conjugation | Unconjugated |
MDC1, also named as KIAA0170 or NFBD1, is a 2089 amino acid protein, which contains two BRCT domains and one FHA domain. MDC1 localizes in the nucleus and is highly expressed in testis. MDC1 is required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. MDC1 may serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked. Protocols Product Specific Protocols IF protocol for MDC1 antibody 83496-1-RR Download protocol WB protocol for MDC1 antibody 83496-1-RR Download protocol Standard Protocols Click here to view our Standard Protocols
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