METTL1 Recombinant monoclonal antibody 84272-1-RR proteintech
$149.00
In stock
SKU
84272-1-RR
| Catalog Number | 84272-1-RR |
| Synonyms | 241108G8, C12orf1, EC:2.1.1.-, EC:2.1.1.33, methyltransferase like 1 |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, FC (Intra), ELISA |
| Applications | WB, IF/ICC, FC (Intra), ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | A549 cells, HeLa cells, HepG2 cells, Caco-2 cells, HuH-7 cells, mouse brain tissue |
| Tested Applications — Positive IF/ICC detected in | A549 cells |
| Tested Applications — Positive FC (Intra) detected in | A431 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Positive WB detected in | A549 cells, HeLa cells, HepG2 cells, Caco-2 cells, HuH-7 cells, mouse brain tissue |
| Positive IF/ICC detected in | A549 cells |
| Positive FC (Intra) detected in | A431 cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Ag7055 Product name: Recombinant human METTL1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-276 aa of BC000550 Sequence: MAAETRNVAGAEAPPPQKRYYRQRAHSNPMADHTLRYPVKPEEMDWSELYPEFFAPLTQNQSHDDPKDKKEKRAQAQVEFADIGCGYGGLLVELSPLFPDTLILGLEIRVKVSDYVQDRIRALRAAPAGGFQNIACLRSNAMKHLPNFFYKGQLTKMFFLFPDPHFKRTKHKWRIISPTLLAEYAYVLRVGGLVYTITDVLELHDWMCTHFEEHPLFERVPLEDLSEDPVVGHLGTSTEEGKKVLRNGGKNFPAIFRRIQDPVLQAVTSQTSLPGH Predict reactive species |
| Full Name | methyltransferase like 1 |
| Calculated Molecular Weight | 31 kDa |
| Observed Molecular Weight | 31-35 kDa |
| GenBank Accession Number | BC000550 |
| Gene Symbol | METTL1 |
| Gene ID (NCBI) | 4234 |
| RRID | AB_3671818 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q9UBP6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for METTL1 antibody 84272-1-RR | Download protocol |
| WB protocol for METTL1 antibody 84272-1-RR | Download protocol |
| human, mouse | WB, IHC, IF Metabolism METTL1-mediated m7G methylation of FoxO1 regulates lipid metabolism in metabolic dysfunction-associated fatty liver disease. Authors - Jiang Du KO Validated View Article |
| mouse | WB Gene Mettl1 mitigates sepsis-induced cardiomyopathy via inhibition of FDX1-dependent cuproptosis. Authors - Wei Siang KD Validated View Article |
| Citations | 2 |
| Dilutions | WB : 1:5000-1:50000 IF/ICC : 1:250-1:1000 FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 241108G8 |
| Conjugation | Unconjugated |
METTL1 methyltransferase mediates m7G methylation within miRNAs and regulates cell migration via its catalytic activity. METTL1 can be inactivated by phosphorylation at Ser27 by protein kinase B (PKBα). Overexpression of METTL1 is widely observed among human cancers. It is also crucial for the regulation of chemoresistance in cancer treatment. In addition, mutations in the human N7-methylguanosine (m7G) methyltransferase complex METTL1/WDR4 cause primordial dwarfism and brain malformation. Protocols Product Specific Protocols IF protocol for METTL1 antibody 84272-1-RR Download protocol WB protocol for METTL1 antibody 84272-1-RR Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- METTL1-mediated m7G methylation of FoxO1 regulates lipid metabolism in metabolic dysfunction-associated fatty liver disease.
Journal: Metabolism | Authors: Jiang Du KO Validated | Species: human, mouse | Application: WB, IHC, IF - Mettl1 mitigates sepsis-induced cardiomyopathy via inhibition of FDX1-dependent cuproptosis.
Journal: Gene | Authors: Wei Siang KD Validated | Species: mouse | Application: WB
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