JAM2 Polyclonal antibody 34226-1-AP proteintech
$149.00
In stock
SKU
34226-1-AP
| Catalog Number | 34226-1-AP |
| Synonyms | CD322, JAM-2, JAM-B, Junctional adhesion molecule 2, Junctional adhesion molecule B |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF-P, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | mouse brain tissue, rat brain tissue |
| Tested Applications — Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF-P detected in | human placenta tissue |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:1000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human placenta tissue |
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Tested Reactivity | human, mouse, rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Eg6328 Product name: Recombinant Mouse JAM2/CD322 protein (rFc Tag) Source: mammalian cells -derived, V37 Tag: C-rFc Domain: 29-236 aa of NM_023844.5 Sequence: FSASKDHRQEVTVIEFQEAILACKTPKKTTSSRLEWKKVGQGVSLVYYQQALQGDFKDRAEMIDFNIRIKNVTRSDAGEYRCEVSAPTEQGQNLQEDKVMLEVLVAPAVPACEVPTSVMTGSVVELRCQDKEGNPAPEYIWFKDGTSLLGNPKGGTHNNSSYTMNTKSGILQFNMISKMDSGEYYCEARNSVGHRRCPGKRMQVDVLN Predict reactive species |
| Full Name | junction adhesion molecule 2 |
| Calculated Molecular Weight | 33 kDa |
| Observed Molecular Weight | 38 kDa |
| GenBank Accession Number | NM_023844.5 |
| Gene Symbol | Jam2 |
| Gene ID (NCBI) | 67374 |
| RRID | AB_3743174 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q9JI59 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for JAM2 antibody 34226-1-AP | Download protocol |
| IHC protocol for JAM2 antibody 34226-1-AP | Download protocol |
| WB protocol for JAM2 antibody 34226-1-AP | Download protocol |
| Citations | - |
| Dilutions | WB : 1:500-1:1000 IHC : 1:50-1:500 IF-P : 1:200-1:800 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Junctional adhesion molecules (JAMs) are integral membrane proteins belonging to the immunoglobulin (Ig) superfamily. JAMs are expressed by leukocytes, platelets, endothelial, and epithelial cells and localized at the tight junction of polarized cells and on the cell surface of leukocytes. JAM-2, also known as VE-JAM or JAM-B, is specifically expressed in lymphatic endothelial cells and endothelial venules. JAM2 regulates cell-cell adhesion and signaling by interacting with other tight junction proteins such as PAR-3 and ZO-1 (PMID: 14657270; 24595739; 11739175; 12953056). Protocols Product Specific Protocols IF protocol for JAM2 antibody 34226-1-AP Download protocol IHC protocol for JAM2 antibody 34226-1-AP Download protocol WB protocol for JAM2 antibody 34226-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
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