PSIP1 Recombinant monoclonal antibody 85395-1-RR proteintech
$149.00
In stock
SKU
85395-1-RR
| Catalog Number | 85395-1-RR |
| Synonyms | 242841C5, CLL associated antigen KW 7, CLL-associated antigen KW-7, DFS70, LEDGF |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, FC (Intra), ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HeLa cells, Jurkat cells. Daudi cells, HEK-293T cells, K-562 cells, NIH/3T3 cells, C6 cells |
| Tested Applications — Positive IHC detected in | human liver cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HepG2 cells |
| Tested Applications — Positive FC (Intra) detected in | HeLa cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Positive WB detected in | HeLa cells, Jurkat cells. Daudi cells, HEK-293T cells, K-562 cells, NIH/3T3 cells, C6 cells |
| Positive IHC detected in | human liver cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HeLa cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human, mouse, rat |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Ag21886 Product name: Recombinant human PSIP1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 104-324 aa of BC064135 Sequence: ASSDVEVEEKETSVSKEDTDHEEKASNEDVTKAVDITTPKAARRGRKRKAGVVTTATASVNLKVSPKRGRPAATEVKIPKPRGRPKMVKQPCPSESDIITEEDKSKKKGQEEKQPKKQPKKDEEGQKEEDKPRKEPDKKEGKKEVESKRKNLAKTGVTSTSDSEEEGDDQEGEKKRKGGRNFQTAHRRNMLKGQHEKEAADRKRKQEEQMETEHQTTCNLQ Predict reactive species |
| Full Name | PC4 and SFRS1 interacting protein 1 |
| Calculated Molecular Weight | 530 aa, 60 kDa |
| Observed Molecular Weight | 75 kDa |
| GenBank Accession Number | BC064135 |
| Gene Symbol | PSIP1 |
| Gene ID (NCBI) | 11168 |
| RRID | AB_3744193 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | O75475 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| FC protocol for PSIP1 antibody 85395-1-RR | Download protocol |
| IF protocol for PSIP1 antibody 85395-1-RR | Download protocol |
| IHC protocol for PSIP1 antibody 85395-1-RR | Download protocol |
| WB protocol for PSIP1 antibody 85395-1-RR | Download protocol |
| Citations | - |
| Dilutions | WB : 1:5000-1:50000 IHC : 1:500-1:2000 IF/ICC : 1:250-1:1000 FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 242841C5 |
| Conjugation | Unconjugated |
PSIP1, also known as PC4 and SRSF1 Interacting Protein 1 or Lens Epithelium-Derived Growth Factor (LEDGF), is a transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. It also plays a role in lens epithelial cell gene regulation and stress responses. It is a chromatin-associated protein with histone chaperone activity, involved in transcriptional elongation. PSIP1 may act as an adapter to coordinate pre-mRNA splicing. PSIP1 encodes two protein isoforms with molecular masses of 52 and 75 kDa. p52 and p75 were identified as interacting with transcription factor PC4 and shown to act as transcriptional coactivators. Protocols Product Specific Protocols FC protocol for PSIP1 antibody 85395-1-RR Download protocol IF protocol for PSIP1 antibody 85395-1-RR Download protocol IHC protocol for PSIP1 antibody 85395-1-RR Download protocol WB protocol for PSIP1 antibody 85395-1-RR Download protocol Standard Protocols Click here to view our Standard Protocols
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