| Catalog Number | 80721-1-RR |
| Synonyms | Synaptopodin, 4K5, KIAA1029, snaptopodin |
| Host | Rabbit |
| Reactivity | human, mouse, rat, pig |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | mouse brain tissue, rat brain tissue, pig brain tissue |
| Tested Applications — Positive IHC detected in | human kidney tissue, mouse brain tissue, mouse kidney tissue, rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:17800 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Positive WB detected in | mouse brain tissue, rat brain tissue, pig brain tissue |
| Positive IHC detected in | human kidney tissue, mouse brain tissue, mouse kidney tissue, rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:2000-1:17800 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Tested Reactivity | human, mouse, rat, pig |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Ag31287 Product name: Recombinant human SYNPO protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 336-510 aa of BC146665 Sequence: SPPSYSVLYPSSDPKSSHLKGQAVPASKTGILEESMARRGSRKSMFTFVEKPKVTPNPDLLDLVQTADEKRRQRDQGEVGVEEEPFALGAEASNFQQEPAPRDRASPAAAEEVVPEWASCLKSPRIQAKPKPKPNQNLSEASGKGAELYARRQSRMEKYVIESSSHTPELARCPS Predict reactive species |
| Full Name | synaptopodin |
| Calculated Molecular Weight | 929 aa, 99 kDa |
| Observed Molecular Weight | 100 kDa |
| GenBank Accession Number | BC146665 |
| Gene Symbol | SYNPO |
| Gene ID (NCBI) | 11346 |
| RRID | AB_2918907 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8N3V7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for SYNPO antibody 80721-1-RR | Download protocol |
| WB protocol for SYNPO antibody 80721-1-RR | Download protocol |
| Citations | - |
| Dilutions | WB : 1:2000-1:17800 IHC : 1:500-1:2000 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 4K5 |
| Conjugation | Unconjugated |
Synaptopodin is a actin-associated protein expressed in mature dendritic spines and renal podocytes. Several isoforms of synaptopodin have been reported: the 100 kDa neural form and 110 kDa renal form. 44-45 kDa may represent the degradation product of synaptopodin. As a podocyte specific protein, synaptopodin expression level was decreased in kidney but increased in the urinary podocytes from patients with preeclampsia, indicating that synaptopodin is a useful marker for preeclampsia. (15841212, 10555072) Protocols Product Specific Protocols IHC protocol for SYNPO antibody 80721-1-RR Download protocol WB protocol for SYNPO antibody 80721-1-RR Download protocol Standard Protocols Click here to view our Standard Protocols