LARS2 Polyclonal antibody 17097-1-AP proteintech
$149.00
In stock
SKU
17097-1-AP
| Catalog Number | 17097-1-AP |
| Synonyms | EC:6.1.1.4, KIAA0028, LARS 2, Leucine--tRNA ligase, mitochondrial, Leucyl-tRNA synthetase |
| Host | Rabbit |
| Reactivity | human and More (2) |
| Reactivity | human, mouse, zebrafish |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HepG2 cells, Jurkat cells, MCF-7 cells |
| Tested Applications — Positive IP detected in | Jurkat cells |
| Tested Applications — Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HepG2 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:2000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| Positive WB detected in | HepG2 cells, Jurkat cells, MCF-7 cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, zebrafish |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag10576 Product name: Recombinant human LARS2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 554-903 aa of BC025989 Sequence: VDLYIGGKEHAVMHLFYARFFSHFCHDQKMVKHREPFHKLLAQGLIKGQTFRLPSGQYLQREEVDLTGSVPVHAKTKEKLEVTWEKMSKSKHNGVDPEEVVEQYGIDTIRLYILFAAPPEKDILWDVKTDALPGVLRWQQRLWTLTTRFIEARASGKSPQPQLLSNKEKAEARKLWEYKNSVISQVTTHFTEDFSLNSAISQLMGLSNALSQASQSVILHSPEFEDALCALMVMAAPLAPHVTSEIWAGLALVPRKLCAHYTWDASVLLQAWPAVDPEFLQQPEVVQMAVLINNKACGKIPVPQQVARDQDKVHEFVLQSELGVRLLQGRSIKKSFLSPRTALINFLVQD Predict reactive species |
| Full Name | leucyl-tRNA synthetase 2, mitochondrial |
| Calculated Molecular Weight | 903 aa, 102 kDa |
| Observed Molecular Weight | 102 kDa |
| GenBank Accession Number | BC025989 |
| Gene Symbol | LARS2 |
| Gene ID (NCBI) | 23395 |
| RRID | AB_2132807 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15031 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for LARS2 antibody 17097-1-AP | Download protocol |
| IHC protocol for LARS2 antibody 17097-1-AP | Download protocol |
| IP protocol for LARS2 antibody 17097-1-AP | Download protocol |
| WB protocol for LARS2 antibody 17097-1-AP | Download protocol |
| mouse | WB,IHC Bone Res Mechanism of Piezo1 regulating chondrocyte mitochondrial function and promoting fracture healing through β-catenin/LARS2 signaling pathway Authors - Tao Zhang View Article |
| human | IF EMBO Mol Med The isolated carboxy-terminal domain of human mitochondrial leucyl-tRNA synthetase rescues the pathological phenotype of mitochondrial tRNA mutations in human cells. Authors - Elena Perli View Article |
| zebrafish | WB Nucleic Acids Res Deletion of Mtu1 (Trmu) in zebrafish revealed the essential role of tRNA modification in mitochondrial biogenesis and hearing function. Authors - Qinghai Zhang View Article |
| mouse,human | WB Oxid Med Cell Longev LARS2 Regulates Apoptosis via ROS-Mediated Mitochondrial Dysfunction and Endoplasmic Reticulum Stress in Ovarian Granulosa Cells. Authors - Shujun Feng KD Validated View Article |
| Citations | 14 |
| Dilutions | WB : 1:500-1:2000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:20-1:200 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
LARS2 (Probable leucine--tRNA ligase, mitochondrial) is also named as mitochondrial LEURS. The deduced 903-amino acid protein has an N-terminal mitochondrial targeting signal that is cleaved after residue 39. LARS2 has characteristics of a class I aminoacyl-tRNA synthetase, including a classical Rossmann fold. It belongs to the class-I aminoacyl-tRNA synthetase family. Protocols Product Specific Protocols IF protocol for LARS2 antibody 17097-1-AP Download protocol IHC protocol for LARS2 antibody 17097-1-AP Download protocol IP protocol for LARS2 antibody 17097-1-AP Download protocol WB protocol for LARS2 antibody 17097-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Single-Cell RNA Sequencing Unveils Unique Transcriptomic Signatures of Organ-Specific Endothelial Cells.
Journal: Circulation | Authors: David T Paik | Species: mouse | Application: IF - Mitochondrial Threonyl-tRNA Synthetase TARS2 Is Required for Threonine-Sensitive mTORC1 Activation.
Journal: Mol Cell | Authors: Sung-Hoon Kim | Species: human | Application: WB,IF - Mechanism of Piezo1 regulating chondrocyte mitochondrial function and promoting fracture healing through β-catenin/LARS2 signaling pathway
Journal: Bone Res | Authors: Tao Zhang | Species: mouse | Application: WB,IHC - Deletion of Mtu1 (Trmu) in zebrafish revealed the essential role of tRNA modification in mitochondrial biogenesis and hearing function.
Journal: Nucleic Acids Res | Authors: Qinghai Zhang | Species: zebrafish | Application: WB - The isolated carboxy-terminal domain of human mitochondrial leucyl-tRNA synthetase rescues the pathological phenotype of mitochondrial tRNA mutations in human cells.
Journal: EMBO Mol Med | Authors: Elena Perli | Species: human | Application: IF - LARS2 Regulates Apoptosis via ROS-Mediated Mitochondrial Dysfunction and Endoplasmic Reticulum Stress in Ovarian Granulosa Cells.
Journal: Oxid Med Cell Longev | Authors: Shujun Feng KD Validated | Species: mouse,human | Application: WB - Deletion of Mtu1 (Trmu) in zebrafish revealed the essential role of tRNA modification in mitochondrial biogenesis and hearing function.
Journal: Nucleic Acids Res | Authors: Qinghai Zhang | Species: zebrafish | Application: WB - Single-cell transcriptomic profiling reveals decreased ER protein Reticulon3 drives the progression of renal fibrosis
Journal: Mol Biomed | Authors: Shuai Guo | Species: mouse | Application: WB,IHC - The isolated carboxy-terminal domain of human mitochondrial leucyl-tRNA synthetase rescues the pathological phenotype of mitochondrial tRNA mutations in human cells.
Journal: EMBO Mol Med | Authors: Elena Perli | Species: human | Application: IF - Targeting insulin receptor substrate-1 delays disease progression in a murine model of aniridia-associated keratopathy
Journal: Biomed Pharmacother | Authors: Ava Dashti | Species: mouse - LARS2 Regulates Apoptosis via ROS-Mediated Mitochondrial Dysfunction and Endoplasmic Reticulum Stress in Ovarian Granulosa Cells.
Journal: Oxid Med Cell Longev | Authors: Shujun Feng | Species: mouse,human | Application: WB - Single-Cell Sequencing Analysis Reveals Dysregulated Energy Metabolism and Altered Synaptic Connectivity in the Mouse Retina Following Dark Rearing.
Journal: Invest Ophthalmol Vis Sci | Authors: Zehui Zhu | Species: mouse | Application: IF - Overexpression of mitochondrial histidyl-tRNA synthetase restores mitochondrial dysfunction caused by a deafness-associated tRNAHis mutation.
Journal: J Biol Chem | Authors: Shasha Gong | Species: human | Application: WB - Single-Cell Transcriptional Profiling Reveals Sex and Age Diversity of Gene Expression in Mouse Endothelial Cells.
Journal: Front Genet | Authors: Xianxi Huang | Species: mouse | Application: WB
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