LBP Monoclonal antibody 66181-1-Ig proteintech
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66181-1-Ig
| Catalog Number | 66181-1-Ig |
| Synonyms | LBP |
| Host | Mouse |
| Reactivity | human and More (1) |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, ELISA |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Mouse / IgG2b |
| Tested Applications — Positive WB detected in | Jurkat cells |
| Tested Applications — Positive IHC detected in | human liver cancer tissue, human liver tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:2000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Positive WB detected in | Jurkat cells |
| Positive IHC detected in | human liver cancer tissue, human liver tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag13485 Product name: Recombinant human LBP protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 128-477 aa of BC022256 Sequence: SFDVSVKGISISVNLLLGSESSGRPTVTASSCSSDIADVEVDMSGDLGWLLNLFHNQIESKFQKVLESRICEMIQKSVSSDLQPYLQTLPVTTEIDSFADIDYSLVEAPRATAQMLEVMFKGEIFHRNHRSPVTLLAAVMSLPEEHNKMVYFAISDYVFNTASLVYHEEGYLNFSITDDMIPPDSNIRLTTKSFRPFVPRLARLYPNMNLELQGSVPSAPLLNFSPGNLSVDPYMEIDAFVLLPSSSKEPVFRLSVATNVSATLTFNTSKITGFLKPGKVKVELKESKVGLFNAELLEALLNYYILNTFYPKFNDKLAEGFPLPLLKRVQLYDLGLQIHKDFPSRRDHPP Predict reactive species |
| Full Name | lipopolysaccharide binding protein |
| Calculated Molecular Weight | 481 aa, 53 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC022256 |
| Gene Symbol | LBP |
| Gene ID (NCBI) | 3929 |
| RRID | AB_2881576 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | P18428 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for LBP antibody 66181-1-Ig | Download protocol |
| WB protocol for LBP antibody 66181-1-Ig | Download protocol |
| human | IF J Pediatr Surg Lipopolysaccharide-binding protein as a biomarker in the diagnosis of necrotizing enterocolitis. Authors - Pengjian Zou View Article |
| mouse,human | WB,IF Nat Commun Lipopolysaccharide binding protein resists hepatic oxidative stress by regulating lipid droplet homeostasis Authors - Qilun Zhang View Article |
| Citations | 2 |
| Dilutions | WB : 1:500-1:2000 IHC : 1:250-1:1000 |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Clone Number | 3A7E3 |
| Conjugation | Unconjugated |
LBP (lipopolysaccharide-binding protein) is an acute-phase protein, predominantly synthesized by hepatocytes. It is present in normal serum at concentrations of 5 to 10 μg/ml, rising up to 200 μg/ml 24 h after induction of an acute-phase response. LBP binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria, and acts as an affinity enhancer for CD14, facilitating its association with LPS. (PMID: 9665271; 11598069) Protocols Product Specific Protocols IHC protocol for LBP antibody 66181-1-Ig Download protocol WB protocol for LBP antibody 66181-1-Ig Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Lipopolysaccharide-binding protein as a biomarker in the diagnosis of necrotizing enterocolitis.
Journal: J Pediatr Surg | Authors: Pengjian Zou | Species: human | Application: IF - Lipopolysaccharide binding protein resists hepatic oxidative stress by regulating lipid droplet homeostasis
Journal: Nat Commun | Authors: Qilun Zhang | Species: mouse,human | Application: WB,IF
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