CoraLite® Plus 488-conjugated LIMK1 Monoclonal antibody CL488-67974 proteintech
$479.00
In stock
SKU
CL488-67974
| Catalog Number | CL488-67974 |
| Synonyms | 2B11E9, EC:2.7.11.1, LIM domain kinase 1, LIMK, LIMK 1 |
| Host | Mouse |
| Reactivity | human, mouse, rat, pig, rabbit |
| Form | Liquid |
| Formulation | PBS, Proclin300, BSA, Glycerol |
| Applications | IF/ICC, FC (Intra) |
| Host / Isotype | Mouse / IgG2a |
| Tested Applications — Positive IF/ICC detected in | HeLa cells |
| Tested Applications — Positive FC (Intra) detected in | HeLa cells |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human, mouse, rat, pig, rabbit |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag28862 Product name: Recombinant human LIMK1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 67-222 aa of BC152982 Sequence: DGQLFCKKDYWARYGESCHGCSEQITKGLVMVAGELKYHPECFICLTCGTFIGDGDTYTLVEHSKLYCGHCYYQTVVTPVIEQILPDSPGSHLPHTVTLVSIPASSHGKRGLSVSIDPPHGPPGCGTEHSHTVRVQGVDPGCMSPDVKNSIHVGDR Predict reactive species |
| Full Name | LIM domain kinase 1 |
| Calculated Molecular Weight | 647 aa, 73 kDa |
| Observed Molecular Weight | 65 kDa |
| GenBank Accession Number | BC152982 |
| Gene Symbol | LIMK1 |
| Gene ID (NCBI) | 3984 |
| RRID | AB_3672967 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Excitation Laser | Blue laser (488 nm) |
| Purification Method | Protein A purification |
| UNIPROT ID | P53667 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. |
| FC protocol for CL Plus 488 LIMK1 antibody CL488-67974 | Download protocol |
| IF protocol for CL Plus 488 LIMK1 antibody CL488-67974 | Download protocol |
| Citations | - |
| Dilutions | IF/ICC : 1:50-1:500 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Clone Number | 2B11E9 |
| Conjugation | CoraLite® Plus 488 |
LIMK1,also named LIMK, belongs to the protein kinase superfamily and TKL Ser/Thr protein kinase family. LIMK1 is a protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. LIMK1 may be involved in brain development. The antibody has no cross-reaction with LIMK2. Protocols Product Specific Protocols FC protocol for CL Plus 488 LIMK1 antibody CL488-67974 Download protocol IF protocol for CL Plus 488 LIMK1 antibody CL488-67974 Download protocol Standard Protocols Click here to view our Standard Protocols
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
