LMOD1 Monoclonal antibody 60414-1-Ig proteintech
$149.00
In stock
SKU
60414-1-Ig
| Catalog Number | 60414-1-Ig |
| Synonyms | Thyroid-associated ophthalmopathy autoantigen, SM-Lmod, Leiomodin-1, Leiomodin1, 1D6H2 |
| Host | Mouse |
| Reactivity | human, mouse, rat, pig, rabbit |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF-P, ELISA |
| Host / Isotype | Mouse / IgG1 |
| Tested Applications — Positive WB detected in | pig colon tissue, ARPE-19 cells, hTERT-RPE1 cells, rabbit colon tissue, rat colon tissue, mouse colon tissue, rabbit testis tissue, rat uterus tissue, mouse uterus tissue |
| Tested Applications — Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF-P detected in | mouse heart tissue |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Recommended Dilutions — Immunofluorescence (IF)-P | IF-P : 1:1000-1:4000 |
| Positive WB detected in | pig colon tissue, ARPE-19 cells, hTERT-RPE1 cells, rabbit colon tissue, rat colon tissue, mouse colon tissue, rabbit testis tissue, rat uterus tissue, mouse uterus tissue |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:1000-1:4000 |
| Tested Reactivity | human, mouse, rat, pig, rabbit |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag7568 Product name: Recombinant human LMOD1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-269 aa of BC001755 Sequence: MEELEKELDVVDPDGSVPVGLRQRNQTEKQSTGVYNREAMLNFCEKETKKLMQREMSMDESKQVETKTDAKNGEERGRDASKKALGPRRDSDLGKEPKRGGLKKSFSRDRDEAGGKSGEKPKEEKIIRGIDKGRVRAAVDKKEAGKDGRGEERAVATKKEEEKKGSDRNTGLSRDKDKKREEMKEVAKKEDDEKVKGGAPAAPPPPPPPLAPPLIMENLKNSLSPATQRKMGDKVLPAQEKNSRDQLLAAIRSSNLKQLKKVEVPKLLQ Predict reactive species |
| Full Name | leiomodin 1 (smooth muscle) |
| Calculated Molecular Weight | 67 kDa |
| Observed Molecular Weight | 75 kDa |
| GenBank Accession Number | BC001755 |
| Gene Symbol | LMOD1 |
| Gene ID (NCBI) | 25802 |
| RRID | AB_3670257 |
| Conjugate | Unconjugated |
| Purification Method | Protein G purification |
| UNIPROT ID | P29536 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for LMOD1 antibody 60414-1-Ig | Download protocol |
| IHC protocol for LMOD1 antibody 60414-1-Ig | Download protocol |
| WB protocol for LMOD1 antibody 60414-1-Ig | Download protocol |
| Citations | - |
| Dilutions | WB : 1:5000-1:50000 IHC : 1:500-1:2000 IF-P : 1:1000-1:4000 |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Clone Number | 1D6H2 |
| Conjugation | Unconjugated |
LMOD1 was initially identified as a 64-kDa autoantigen in an expression screen with sera from patients with Hashimoto's thyroiditis. Recently immunohistochemistry and immunoblotting of LMOD1 demonstrated its highly restrictive pattern of expression in SMC lineages,like adult aorta, and bladder. LMOD1 is also shown to exhibit an embryonic and adult expression pattern. These data identify LMOD1 as a new SMC-restricted gene associated with the SMC differentiated phenotype. Protocols Product Specific Protocols IF protocol for LMOD1 antibody 60414-1-Ig Download protocol IHC protocol for LMOD1 antibody 60414-1-Ig Download protocol WB protocol for LMOD1 antibody 60414-1-Ig Download protocol Standard Protocols Click here to view our Standard Protocols
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