TNFSF11/RANKL Recombinant monoclonal antibody 84431-5-RR proteintech
$149.00
In stock
SKU
84431-5-RR
| Catalog Number | 84431-5-RR |
| Synonyms | 241791B7, CD254, ODF, OPGL, Osteoclast differentiation factor |
| Host | Rabbit |
| Reactivity | mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | mouse spleen tissue, mouse liver tissue |
| Tested Applications — Positive IHC detected in | mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:4000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Positive WB detected in | mouse spleen tissue, mouse liver tissue |
| Positive IHC detected in | mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Tested Reactivity | mouse |
| Cited Reactivity | mouse |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Eg1956 Product name: Recombinant Mouse TNFSF11/RANKL protein (rFc Tag)(HPLC verified) Source: mammalian cells -derived, pHZ-KIsec-N-rFc Tag: N-rFc Domain: 72-316 aa of NM_011613.3 Sequence: RAQMDPNRISEDSTHCFYRILRLHENADLQDSTLESEDTLPDSCRRMKQAFQGAVQKELQHIVGPQRFSGAPAMMEGSWLDVAQRGKPEAQPFAHLTINAASIPSGSHKVTLSSWYHDRGWAKISNMTLSNGKLRVNQDGFYYLYANICFRHHETSGSVPTDYLQLMVYVVKTSIKIPSSHNLMKGGSTKNWSGNSEFHFYSINVGGFFKLRAGEEISIQVSNPSLLDPDQDATYFGAFKVQDID Predict reactive species |
| Full Name | tumor necrosis factor (ligand) superfamily, member 11 |
| Calculated Molecular Weight | 35KD |
| Observed Molecular Weight | 22 kDa |
| GenBank Accession Number | NM_011613.3 |
| Gene Symbol | Tnfsf11 |
| Gene ID (NCBI) | 21943 |
| RRID | AB_3671958 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purfication |
| UNIPROT ID | O35235 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for TNFSF11/RANKL antibody 84431-5-RR | Download protocol |
| WB protocol for TNFSF11/RANKL antibody 84431-5-RR | Download protocol |
| mouse | WB Mol Immunol SMAD7-mediated ferroptosis in macrophages drives osteoporosis progression: A multi-omics study. Authors - Qianning Li View Article |
| Citations | 3 |
| Dilutions | WB : 1:1000-1:4000 IHC : 1:400-1:1600 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 241791B7 |
| Conjugation | Unconjugated |
TNFSF11, known as Tumor Necrosis Factor Superfamily Member 11, also known as RANKL (Receptor Activator of Nuclear Factor-κB Ligand), TRANCE, etc., is a member of the Tumor Necrosis Factor (TNF) superfamily.RANKL is a type II homotrimeric transmembrane protein located primarily at the plasma membrane. RANKL is a type II homotrimeric transmembrane protein located mainly at the plasma membrane, and in addition to the membrane-bound form (mRNAKL), it can also be cleaved by proteolytic cleavage of the cell membrane to produce the soluble form (sRANKL). The membrane-bound form of RANKL is the main form involved in triggering the RANKL/RANK signaling pathway and mediating osteoclastogenesis. Current studies have shown that TNFSF11 acts primarily in the immune and skeletal systems and has been implicated in the pathogenesis of degenerative bone diseases, carcinogenesis, and asthma. Protocols Product Specific Protocols IHC protocol for TNFSF11/RANKL antibody 84431-5-RR Download protocol WB protocol for TNFSF11/RANKL antibody 84431-5-RR Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- KPT-330 alleviates osteoarthritis by inhibiting subchondral bone osteoclastogenesis via the NF-κB and MAPK signaling pathways.
Journal: iScience | Authors: Wugui Chen - Activated NETosis of bone marrow neutrophils up-regulates macrophage osteoclastogenesis via cGAS-STING/AKT2 pathway to promote osteoporosis
Journal: Exp Cell Res | Authors: Yutong Guo - SMAD7-mediated ferroptosis in macrophages drives osteoporosis progression: A multi-omics study.
Journal: Mol Immunol | Authors: Qianning Li | Species: mouse | Application: WB
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