MAFB Polyclonal antibody 20189-1-AP proteintech
$149.00
In stock
SKU
20189-1-AP
| Catalog Number | 20189-1-AP |
| Synonyms | KRML, Maf B, Maf-B, Transcription factor MafB, V-maf musculoaponeurotic fibrosarcoma oncogene homolog B |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, FC (Intra), IP, ChIP, ELISA |
| Applications | WB, IHC, IF/ICC, FC (Intra), ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | RAW 264.7 cells |
| Tested Applications — Positive IHC detected in | mouse pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | U-251 cells |
| Tested Applications — Positive FC (Intra) detected in | RAW 264.7 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:2000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Positive WB detected in | RAW 264.7 cells |
| Positive IHC detected in | mouse pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U-251 cells |
| Positive FC (Intra) detected in | RAW 264.7 cells |
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag14102 Product name: Recombinant human MAFB protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 168-323 aa of BC036689 Sequence: QASPPPSSAASPAQQLPTSHPGPGPHATASATAAGGNGSVEDRFSDDQLVSMSVRELNRHLRGFTKDEVIRLKQKRRTLKNRGYAQSCRYKRVQQKHHLENEKTQLIQQVEQLKQEVSRLARERDAYKVKCEKLANSGFREAGSTSDSPSSPEFFL Predict reactive species |
| Full Name | v-maf musculoaponeurotic fibrosarcoma oncogene homolog B (avian) |
| Calculated Molecular Weight | 323 aa, 36 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC036689 |
| Gene Symbol | MAFB |
| Gene ID (NCBI) | 9935 |
| ENSEMBL Gene ID | ENSG00000204103 |
| RRID | AB_2878654 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y5Q3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| FC protocol for MAFB antibody 20189-1-AP | Download protocol |
| IF protocol for MAFB antibody 20189-1-AP | Download protocol |
| IHC protocol for MAFB antibody 20189-1-AP | Download protocol |
| WB protocol for MAFB antibody 20189-1-AP | Download protocol |
| mouse | WB,IP Commun Biol MafB regulates NLRP3 inflammasome activation by sustaining p62 expression in macrophages Authors - Huachun Cui View Article |
| human | WB Front Microbiol Transcription factor MAFB controls type I and II interferon response-mediated host immunity in Mycobacterium tuberculosis-infected macrophages Authors - Haruka Hikichi KD Validated View Article |
| rat | WB Mol Cell Endocrinol lnc-HC ameliorates steatosis by promoting miR-130b-3p biogenesis and the assembly of an RNA-induced silencing complex Authors - Xi Lan KD Validated View Article |
| Citations | 9 |
| Dilutions | WB : 1:500-1:2000 IHC : 1:250-1:1000 IF/ICC : 1:500-1:2000 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
MAFB (MAF BZIP Transcription Factor B), also known as V-maf musculoaponeurotic fibrosarcoma oncogene homolog B, is a widely expressed basic leucine zipper transcription factor belonging to the Maf family. MAFB expression is significantly elevated in response to a broad range of metabolic and immunological stimuli that promote macrophage M2 polarization, cholesterol efflux, or both, whereas it is strongly down-regulated in response to various pro-inflammatory pathogenic triggers(PMID: 28790455). MAFB is highly expressed in tumor tissues and required for proliferation and tumorigenicity of osteosarcoma cells(PMID: 32234710) and is a marker of tumor-associated macrophages in both mouse and human(PMID: 31679694). Protocols Product Specific Protocols FC protocol for MAFB antibody 20189-1-AP Download protocol IF protocol for MAFB antibody 20189-1-AP Download protocol IHC protocol for MAFB antibody 20189-1-AP Download protocol WB protocol for MAFB antibody 20189-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Glia maturation factor-γ regulates amyloid-β42 phagocytosis through scavenger receptor AI in murine macrophages
Journal: J Leukoc Biol | Authors: Wulin Aerbajinai | Species: mouse | Application: WB,IF - Musashi2 promotes EGF-induced EMT in pancreatic cancer via ZEB1-ERK/MAPK signaling
Journal: J Exp Clin Cancer Res | Authors: Weiwei Sheng | Species: human | Application: WB - Profibrotic macrophage-derived CXCL4 promotes pericyte-to-myofibroblast transition after spinal cord injury.
Journal: J Orthop Translat | Authors: Gang Li | Species: mouse | Application: ChIP - Transcription factor MAFB controls type I and II interferon response-mediated host immunity in Mycobacterium tuberculosis-infected macrophages
Journal: Front Microbiol | Authors: Haruka Hikichi KD Validated | Species: human | Application: WB - lnc-HC ameliorates steatosis by promoting miR-130b-3p biogenesis and the assembly of an RNA-induced silencing complex
Journal: Mol Cell Endocrinol | Authors: Xi Lan KD Validated | Species: rat | Application: WB - MafB regulates NLRP3 inflammasome activation by sustaining p62 expression in macrophages
Journal: Commun Biol | Authors: Huachun Cui | Species: mouse | Application: WB,IP
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
