MAP3K1 Polyclonal antibody 19970-1-AP proteintech
$149.00
In stock
SKU
19970-1-AP
| Catalog Number | 19970-1-AP |
| Synonyms | MEKK, MEK kinase 1, MAPKKK1, MAPK/ERK kinase kinase 1, EC:2.7.11.25 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | mouse embryo tissue, A431 cells, mouse spleen tissue, A549 cells, NIH/3T3 cells, rat spleen tissue |
| Tested Applications — Positive IP detected in | NIH/3T3 cells |
| Tested Applications — Positive IHC detected in | human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HeLa cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:4000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| Positive WB detected in | mouse embryo tissue, A431 cells, mouse spleen tissue, A549 cells, NIH/3T3 cells, rat spleen tissue |
| Positive IP detected in | NIH/3T3 cells |
| Positive IHC detected in | human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide Predict reactive species |
| Full Name | mitogen-activated protein kinase kinase kinase 1 |
| Calculated Molecular Weight | 164 kDa |
| Observed Molecular Weight | 164 kDa |
| GenBank Accession Number | NM_005921 |
| Gene Symbol | MAP3K1 |
| Gene ID (NCBI) | 4214 |
| RRID | AB_2878633 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13233 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for MAP3K1 antibody 19970-1-AP | Download protocol |
| IHC protocol for MAP3K1 antibody 19970-1-AP | Download protocol |
| IP protocol for MAP3K1 antibody 19970-1-AP | Download protocol |
| WB protocol for MAP3K1 antibody 19970-1-AP | Download protocol |
| human | WB Cell Death Dis LncRNA KCNQ1OT1 activated by c-Myc promotes cell proliferation via interacting with FUS to stabilize MAP3K1 in acute promyelocytic leukemia. Authors - Doudou Tang View Article |
| mouse | WB,IF Biomed Pharmacother Xihuang pill promotes apoptosis of Treg cells in the tumor microenvironment in 4T1 mouse breast cancer by upregulating MEKK1/SEK1/JNK1/AP-1 pathway. Authors - Liang Su View Article |
| Human | Biomed Res Int Next-Generation Sequencing Panel Analysis of Clinically Relevant Mutations in Circulating Cell-Free DNA from Patients with Gestational Trophoblastic Neoplasia: A Pilot Study. Authors - Lingxiao Luo View Article |
| Citations | 18 |
| Dilutions | WB : 1:1000-1:4000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:20-1:200 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
MAP3K1, also named as MAPKKK1, MEKK and MEKK1, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and MAP kinase kinase kinase subfamily. MAP3K1 is a component of a protein kinase signal transduction cascade. MAP3K1 activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4. It activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway. It catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein. The antibody recognizes the N-term of MAP3K1. Protocols Product Specific Protocols IF protocol for MAP3K1 antibody 19970-1-AP Download protocol IHC protocol for MAP3K1 antibody 19970-1-AP Download protocol IP protocol for MAP3K1 antibody 19970-1-AP Download protocol WB protocol for MAP3K1 antibody 19970-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Circulating miR-320b Contributes to CD4+ T-Cell Proliferation in Systemic Lupus Erythematosus via MAP3K1
Journal: J Immunol Res | Authors: Zutong Li | Species: human | Application: WB - LncRNA SLCO4A1-AS1 predicts poor prognosis and promotes proliferation and metastasis via the EGFR/MAPK pathway in colorectal cancer.
Journal: Int J Biol Sci | Authors: Rui Tang | Species: human | Application: WB - Extracellular vesicles secreted from mesenchymal stem cells exert anti-apoptotic and anti-inflammatory effects via transmitting microRNA-18b in rats with diabetic retinopathy.
Journal: Int Immunopharmacol | Authors: Zepeng Xu | Species: human | Application: WB - Xihuang pill promotes apoptosis of Treg cells in the tumor microenvironment in 4T1 mouse breast cancer by upregulating MEKK1/SEK1/JNK1/AP-1 pathway.
Journal: Biomed Pharmacother | Authors: Liang Su | Species: mouse | Application: WB,IF - LncRNA KCNQ1OT1 activated by c-Myc promotes cell proliferation via interacting with FUS to stabilize MAP3K1 in acute promyelocytic leukemia.
Journal: Cell Death Dis | Authors: Doudou Tang | Species: human | Application: WB - Next-Generation Sequencing Panel Analysis of Clinically Relevant Mutations in Circulating Cell-Free DNA from Patients with Gestational Trophoblastic Neoplasia: A Pilot Study.
Journal: Biomed Res Int | Authors: Lingxiao Luo | Species: Human
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