MAPKAPK2 Polyclonal antibody 13949-1-AP proteintech
$149.00
In stock
SKU
13949-1-AP
| Catalog Number | 13949-1-AP |
| Synonyms | MAPKAP-K2, MAPKAPK 2, MAPKAP kinase 2, MAPK-activated protein kinase 2, MAP kinase-activated protein kinase 2 |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HeLa cells, A549 cells, mouse skeletal muscle tissue, mouse colon tissue |
| Tested Applications — Positive IHC detected in | human breast cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HeLa cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:1000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Positive WB detected in | HeLa cells, A549 cells, mouse skeletal muscle tissue, mouse colon tissue |
| Positive IHC detected in | human breast cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag5060 Product name: Recombinant human MAPKAPK2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-400 aa of BC036060 Sequence: MLSNSQGQSPPVPFPAPAPPPQPPTPALPHPPAQPPPPPPQQFPQFHVKSGLQIKKNAIIDDYKVTSQVLGLGINGKVLQIFNKRTQEKFALKMLQDCPKARREVELHWRASQCPHIVRIVDVYENLYAGRKCLLIVMECLDGGELFSRIQDRGDQAFTEREASEIMKSIGEAIQYLHSINIAHRDVKPENLLYTSKRPNAILKLTDFGFAKETTSHNSLTTPCYTPYYVAPEVLGPEKYDKSCDMWSLGVIMYILLCGYPPFYSNHGLAISPGMKTRIRMGQYEFPNPEWSEVSEEVKMLIRNLLKTEPTQRMTITEFMNHPWIMQSTKVPQTPLHTSRVLKEDKERWEDVKEEMTSALATMRVDYEQIKIKKIEDASNPLLLKRRKKARALEAAALAH Predict reactive species |
| Full Name | mitogen-activated protein kinase-activated protein kinase 2 |
| Calculated Molecular Weight | 400 aa, 46 kDa |
| Observed Molecular Weight | 47-50 kDa |
| GenBank Accession Number | BC036060 |
| Gene Symbol | MAPKAPK2 |
| Gene ID (NCBI) | 9261 |
| RRID | AB_2877995 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P49137 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for MAPKAPK2 antibody 13949-1-AP | Download protocol |
| IHC protocol for MAPKAPK2 antibody 13949-1-AP | Download protocol |
| WB protocol for MAPKAPK2 antibody 13949-1-AP | Download protocol |
| human | WB,IHC Cell Death Dis Inhibition of DTYMK significantly restrains the growth of HCC and increases sensitivity to oxaliplatin. Authors - Fengze Sun View Article |
| mouse | WB Brain Res Bull The p38 mitogen activated protein kinase regulates β-amyloid protein internalization through the α7 nicotinic acetylcholine receptor in mouse brain. Authors - Kai-Ge Ma View Article |
| Citations | 15 |
| Dilutions | WB : 1:500-1:1000 IHC : 1:50-1:500 IF/ICC : 1:200-1:800 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
MAPKAPK2(mitogen-activated protein kinase-activated protein kinase 2) is also named as MK2, MAPKAP-K2, MK-2 and belongs to the CAMK Ser/Thr protein kinase family. MAPKAPK2, one of several kinases directly phosphorylated and activated by p38 MAPK, plays a central role in the inflammatory response and is in the nucleus of unstimulated cells and moves rapidly to the cytoplasm after stimulation(PMID:12171911). It is also involved in many other cellular processes including stress responses, nuclear export, gene expression regulation and cell proliferation. Multiple residues of MAPKAPK2 are generally phosphorylated in vivo in response to stress, but only 4 residues(Thr25, Thr222, Ser272, and Thr334) are phosphorylated by p38 MAPK in vitro(PMID:22351694). It has 2 isoforms produced by alternative splicing and the range of the molecular weight is 42-60 kDa according to the references(PMID:10666409; 11328854;8995385). Protocols Product Specific Protocols IF protocol for MAPKAPK2 antibody 13949-1-AP Download protocol IHC protocol for MAPKAPK2 antibody 13949-1-AP Download protocol WB protocol for MAPKAPK2 antibody 13949-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Sophoridine inhibits human colorectal cancer progression via targeting MAPKAPK2.
Journal: Mol Cancer Res | Authors: Rui Wang | Species: human | Application: WB - L-Theanine mediates the p38MAPK signaling pathway to alleviate heat-induced oxidative stress and inflammation in mice.
Journal: Food Funct | Authors: Kehong Liu | Species: mouse | Application: WB - p38 MAPK-DRP1 signaling is involved in mitochondrial dysfunction and cell death in mutant A53T α-synuclein model of Parkinson's disease.
Journal: Toxicol Appl Pharmacol | Authors: Chen Gui | Species: human | Application: WB - Aryl hydrocarbon receptor inhibits inflammation in DSS‑induced colitis via the MK2/p‑MK2/TTP pathway.
Journal: Int J Mol Med | Authors: Qimeng Wang | Species: human | Application: WB - The p38 mitogen activated protein kinase regulates β-amyloid protein internalization through the α7 nicotinic acetylcholine receptor in mouse brain.
Journal: Brain Res Bull | Authors: Kai-Ge Ma | Species: mouse | Application: WB - Inhibition of DTYMK significantly restrains the growth of HCC and increases sensitivity to oxaliplatin.
Journal: Cell Death Dis | Authors: Fengze Sun | Species: human | Application: WB,IHC
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