Anti-Human CD146/MCAM Rabbit Recombinant Antibody 98561-2-RR proteintech
$75.00
In stock
SKU
98561-2-RR
| Catalog Number | 98561-2-RR |
| Synonyms | CD146, MCAM, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, MUC18 |
| Host | Rabbit |
| Reactivity | human |
| Form | Liquid |
| Formulation | PBS, Azide |
| Applications | IHC, FC |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive FC detected in | HeLa cells |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | HeLa cells |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Eg2938 Product name: Recombinant Human CD146/MCAM protein (rFc Tag) Source: mammalian cells -derived, V37 Tag: C-rFc Domain: 24-559 aa of NM_006500.2 Sequence: VPGEAEQPAPELVEVEVGSTALLKCGLSQSQGNLSHVDWFSVHKEKRTLIFRVRQGQGQSEPGEYEQRLSLQDRGATLALTQVTPQDERIFLCQGKRPRSQEYRIQLRVYKAPEEPNIQVNPLGIPVNSKEPEEVATCVGRNGYPIPQVIWYKNGRPLKEEKNRVHIQSSQTVESSGLYTLQSILKAQLVKEDKDAQFYCELNYRLPSGNHMKESREVTVPVFYPTEKVWLEVEPVGMLKEGDRVEIRCLADGNPPPHFSISKQNPSTREAEEETTNDNGVLVLEPARKEHSGRYECQGLDLDTMISLLSEPQELLVNYVSDVRVSPAAPERQEGSSLTLTCEAESSQDLEFQWLREETGQVLERGPVLQLHDLKREAGGGYRCVASVPSIPGLNRTQLVNVAIFGPPWMAFKERKVWVKENMVLNLSCEASGHPRPTISWNVNGTASEQDQDPQRVLSTLNVLVTPELLETGVECTASNDLGKNTSILFLELVNLTTLTPDSNTTTGLSTSTASPHTRANSTSTERKLPEPESRG Predict reactive species |
| Full Name | melanoma cell adhesion molecule |
| Calculated Molecular Weight | 72 kDa |
| GenBank Accession Number | NM_006500.2 |
| Gene Symbol | CD146 |
| Gene ID (NCBI) | 4162 |
| RRID | AB_3746180 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | P43121-1 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2 - 8°C. Stable for one year after shipment. |
| FC protocol for CD146/MCAM antibody 98561-2-RR | Download protocol |
| IHC protocol for CD146/MCAM antibody 98561-2-RR | Download protocol |
| Citations | - |
| Dilutions | IHC : 1:500-1:2000 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 250530B2 |
| Conjugation | Unconjugated |
CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988). Protocols Product Specific Protocols FC protocol for CD146/MCAM antibody 98561-2-RR Download protocol IHC protocol for CD146/MCAM antibody 98561-2-RR Download protocol Standard Protocols Click here to view our Standard Protocols
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