MCCC1 Polyclonal antibody 30081-1-AP proteintech
$149.00
In stock
SKU
30081-1-AP
| Catalog Number | 30081-1-AP |
| Synonyms | MCCA, MCC B, EC:6.4.1.4, 3-methylcrotonyl-CoA:carbon dioxide ligase subunit alpha, 3-methylcrotonyl-CoA carboxylase biotin-containing subunit |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HepG2 cells, mouse brain tissue, mouse heart tissue, rat brain tissue |
| Tested Applications — Positive IHC detected in | human ovary cancer tissue, human intrahepatic cholangiocarcinoma tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | U-251 cells, MCF-7 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:4000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Positive WB detected in | HepG2 cells, mouse brain tissue, mouse heart tissue, rat brain tissue |
| Positive IHC detected in | human ovary cancer tissue, human intrahepatic cholangiocarcinoma tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U-251 cells, MCF-7 cells |
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag32459 Product name: Recombinant human MCCC1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 42-200 aa of BC004187 Sequence: TTATGRNITKVLIANRGEIACRVMRTAKKLGVQTVAVYSEADRNSMHVDMADEAYSIGPAPSQQSYLSMEKIIQVAKTSAAQAIHPGCGFLSENMEFAELCKQEGIIFIGPPPSAIRDMGIKSTSKSIMAAAGVPVVEGYHGEDQSDQCLKEHARRIGY Predict reactive species |
| Full Name | methylcrotonoyl-Coenzyme A carboxylase 1 (alpha) |
| Calculated Molecular Weight | 76 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC004187 |
| Gene Symbol | MCCC1 |
| Gene ID (NCBI) | 56922 |
| RRID | AB_3086226 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96RQ3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for MCCC1 antibody 30081-1-AP | Download protocol |
| IHC protocol for MCCC1 antibody 30081-1-AP | Download protocol |
| WB protocol for MCCC1 antibody 30081-1-AP | Download protocol |
| Citations | - |
| Dilutions | WB : 1:1000-1:4000 IHC : 1:200-1:800 IF/ICC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
MCCC1 is the large subunit of 3-methylcrotonyl-CoA carboxylase. This enzyme functions as a heterodimer and catalyzes the carboxylation of 3-methylcrotonyl-CoA to form 3-methylglutaconyl-CoA. Mutations in this gene are associated with 3-Methylcrotonylglycinuria, an autosomal recessive disorder of leucine catabolism. Protocols Product Specific Protocols IF protocol for MCCC1 antibody 30081-1-AP Download protocol IHC protocol for MCCC1 antibody 30081-1-AP Download protocol WB protocol for MCCC1 antibody 30081-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
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