METTL2 Polyclonal antibody 16983-1-AP proteintech
$149.00
In stock
SKU
16983-1-AP
| Catalog Number | 16983-1-AP |
| Synonyms | methyltransferase like 2B, METL, METTL2, METTL2A, METTL2A/B, METTL2B, PSENIP1 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | SH-SY5Y cells, MCF-7 cells |
| Tested Applications — Positive IHC detected in | mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | MCF-7 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:6000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Positive WB detected in | SH-SY5Y cells, MCF-7 cells |
| Positive IHC detected in | mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag10410 Product name: Recombinant human METTL2B protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-313 aa of BC107586 Sequence: MPVDYEINAHKYWNDFYKIHENGFFKDRHWLFTEFPELAPSQNQNHLKDWFLENKSEVCECRNNEDGPGLIMEEQHKCSSKSLEHKTQTPPVEENVTQKISDLEICADEFPGSSATYRILEVGCGVGNTVFPILQTNNDPGLFVYCCDFSSTAIELVQTNSEYDPSRCFAFVHDLCDEEKSYPVPKGSLDIIILIFVLSAIVPDKMQKAINRLSRLLKPGGMVLLRDYGRYDMAQLRFKKGQCLSGNFYVRGDGTRVYFFTQEELDTLFTTAGLEKVQNLVDRRLQVNRGKQLTMYRVWIQCKYCKPLLSSTS Predict reactive species |
| Full Name | methyltransferase like 2B |
| Calculated Molecular Weight | 313 aa, 36 kDa |
| Observed Molecular Weight | 36 kDa |
| GenBank Accession Number | BC107586 |
| Gene Symbol | METTL2B |
| Gene ID (NCBI) | 55798 |
| RRID | AB_2923564 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6P1Q9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for METTL2 antibody 16983-1-AP | Download protocol |
| IHC protocol for METTL2 antibody 16983-1-AP | Download protocol |
| WB protocol for METTL2 antibody 16983-1-AP | Download protocol |
| human | IHC J Cell Mol Med Nomogram Based on A-To-I RNA Editing for Predicting Overall Survival in Patients With Breast Cancer Authors - Yangyang Zhang View Article |
| mouse | WB Stem Cell Res Ther Mechanical tension-induced Dalrd3 elevation enhances osteogenic differentiation of bone suture stem cells by upregulating Id3 translation Authors - Jie Chen View Article |
| rat | WB Brain Res Bull Rab3a attenuates spinal cord injury by mediating vesicle release Authors - Yuhao Yang KD Validated View Article |
| Citations | 6 |
| Dilutions | WB : 1:1000-1:6000 IHC : 1:50-1:500 IF/ICC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
METTL2A and METTL2B are required for the threonyl and argininyl tRNA methylation. METTL2 methyltransferase is responsible for 3-methylcytosine modification of arginine tRNAs in mammals. METTL2A and METTL2B are in higher homology 99%. This antibody detects both METTL2A and METTL2B. Protocols Product Specific Protocols IF protocol for METTL2 antibody 16983-1-AP Download protocol IHC protocol for METTL2 antibody 16983-1-AP Download protocol WB protocol for METTL2 antibody 16983-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- m3C32 tRNA modification controls serine codon-biased mRNA translation, cell cycle, and DNA-damage response
Journal: Nat Commun | Authors: Jia Cui KO Validated | Species: human | Application: WB - Mechanical tension-induced Dalrd3 elevation enhances osteogenic differentiation of bone suture stem cells by upregulating Id3 translation
Journal: Stem Cell Res Ther | Authors: Jie Chen | Species: mouse | Application: WB - METTL2B m3C RNA transferase: oncogenic role in ovarian cancer progression via regulation of the mTOR/AKT pathway and its link to the tumor immune microenvironment
Journal: BMC Cancer | Authors: Yizi Meng | Species: human | Application: WB,IHC - Rab3a attenuates spinal cord injury by mediating vesicle release
Journal: Brain Res Bull | Authors: Yuhao Yang KD Validated | Species: rat | Application: WB - Development and Validation of Diagnostic Models for Transcriptomic Signature Genes for Multiple Tissues in Osteoarthritis
Journal: J Inflamm Res | Authors: Qichang Gao | Species: human | Application: IHC - Nomogram Based on A-To-I RNA Editing for Predicting Overall Survival in Patients With Breast Cancer
Journal: J Cell Mol Med | Authors: Yangyang Zhang | Species: human | Application: IHC
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