METTL5 Polyclonal antibody 16791-1-AP proteintech
$149.00
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SKU
16791-1-AP
| Catalog Number | 16791-1-AP |
| Synonyms | EC:2.1.1.-, HSPC133, methyltransferase like 5, Methyltransferase-like protein 5, rRNA N6-adenosine-methyltransferase METTL5 |
| Host | Rabbit |
| Reactivity | human |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IP, RIP, ELISA |
| Applications | WB, IF/ICC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HEK-293 cells, HeLa cells, K-562 cells, PC-3 cells |
| Tested Applications — Positive IP detected in | PC-3 cells |
| Tested Applications — Positive IF/ICC detected in | HepG2 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:12000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Positive WB detected in | HEK-293 cells, HeLa cells, K-562 cells, PC-3 cells |
| Positive IP detected in | PC-3 cells |
| Positive IF/ICC detected in | HepG2 cells |
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag10232 Product name: Recombinant human METTL5 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-205 aa of BC000921 Sequence: MKKVRLKELESRLQQVDGFEKPKLLLEQYPTRPHIAACMLYTIHNTYDDIENKVVADLGCGCGVLSIGTAMLGAGLCVGFDIDEDALEIFNRNAEEFELTNIDMVQCDVCLLSNRMSKSFDTVIMNPPFGTKNNKGTDMAFLKTALEMARTAVYSLHKSSTREHVQKKAAEWKIKIDIIAELRYDLPASYKFHKKKSVDIEVDLI Predict reactive species |
| Full Name | methyltransferase like 5 |
| Calculated Molecular Weight | 209 aa, 24 kDa |
| Observed Molecular Weight | 24-27 kDa |
| GenBank Accession Number | BC000921 |
| Gene Symbol | METTL5 |
| Gene ID (NCBI) | 29081 |
| RRID | AB_2142051 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NRN9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| WB protocol for METTL5 antibody 16791-1-AP | Download protocol |
| IF protocol for METTL5 antibody 16791-1-AP | Download protocol |
| IHC protocol for METTL5 antibody 16791-1-AP | Download protocol |
| IP protocol for METTL5 antibody 16791-1-AP | Download protocol |
| human | WB Cell Rep Ribosome 18S m6A Methyltransferase METTL5 Promotes Translation Initiation and Breast Cancer Cell Growth. Authors - Bowen Rong KO Validated View Article |
| Citations | 26 |
| Dilutions | WB : 1:2000-1:12000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IF/ICC : 1:200-1:800 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
METTL5 is a member of the METTL family of S-adenosyl-methionine-dependent methyltransferases that adds m6A to a specific site, nucleotide A-1832 of 18S rRNA, hereafter referred to as m6A1832. METTL5 is localized in the nucleolus and cytoplasm. Protocols Product Specific Protocols WB protocol for METTL5 antibody 16791-1-AP Download protocol IF protocol for METTL5 antibody 16791-1-AP Download protocol IHC protocol for METTL5 antibody 16791-1-AP Download protocol IP protocol for METTL5 antibody 16791-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- The 18S rRNA m6 A methyltransferase METTL5 promotes mouse embryonic stem cell differentiation.
Journal: EMBO Rep | Authors: Ming Xing KD Validated | Application: WB - The METTL5-TRMT112 N6-methyladenosine methyltransferase complex regulates mRNA translation via 18S rRNA methylation.
Journal: J Biol Chem | Authors: Caraline Sepich-Poore KO Validated | Species: human | Application: WB - Loss of m6A Methyltransferase METTL5 Promotes Cardiac Hypertrophy Through Epitranscriptomic Control of SUZ12 Expression.
Journal: Front Cardiovasc Med | Authors: Yanchuang Han | Application: WB - Long noncoding RNA UBA6-AS1 inhibits the malignancy of ovarian cancer cells via suppressing the decay of UBA6 mRNA.
Journal: Bioengineered | Authors: Yaogang Wang | Species: human | Application: RIP - The potential role of methyltransferase-like 5 in deficient mismatch repair of uterine corpus endometrial carcinoma.
Journal: Bioengineered | Authors: Xiaojuan Liu KD Validated | Species: human | Application: WB,IHC - Ribosome 18S m6A Methyltransferase METTL5 Promotes Translation Initiation and Breast Cancer Cell Growth.
Journal: Cell Rep | Authors: Bowen Rong KO Validated | Species: human | Application: WB - METTL5 triggers the ferroptosis of cardiomyocytes in sepsis-induced myocardial injury
Journal: Free Radic Biol Med | Authors: Xiaoye Wang | Application: WB - Ribosome 18S m6A Methyltransferase METTL5 Promotes Translation Initiation and Breast Cancer Cell Growth.
Journal: Cell Rep | Authors: Bowen Rong | Species: human | Application: WB - IGF2BP3 redirects glycolytic flux to promote one-carbon metabolism and RNA methylation
Journal: Cell Rep | Authors: Gunjan Sharma - METTL5 regulates cranial suture fusion via Wnt signaling
Journal: Fundam Res | Authors: Kexin Lei | Application: IHC - METTL5 deficiency impairs osteogenesis through OSER1-dependent antioxidant regulation.
Journal: JCI Insight | Authors: Kexin Lei | Application: IHC - The 18S rRNA m6 A methyltransferase METTL5 promotes mouse embryonic stem cell differentiation.
Journal: EMBO Rep | Authors: Ming Xing | Application: WB - Scutellarin suppresses ovarian cancer progression by targeting METTL5
Journal: Sci Rep | Authors: Ling Ding | Species: human | Application: WB,IHC - TWIK-related acid-sensitive K+ channel 2 promotes renal fibrosis by inducing cell-cycle arrest
Journal: iScience | Authors: Jian Zhang | Application: WB - ZNF460 Promotes the m6A Modification of COPS6 to Facilitate Immune Escape in Colorectal Cancer.
Journal: FASEB J | Authors: Weijian Lun | Species: human | Application: WB - The METTL5-TRMT112 N6-methyladenosine methyltransferase complex regulates mRNA translation via 18S rRNA methylation.
Journal: J Biol Chem | Authors: Caraline Sepich-Poore | Species: human | Application: WB - The m6A methyltransferase METTL5 promotes neutrophil extracellular trap network release to regulate hepatocellular carcinoma progression
Journal: Cancer Med | Authors: Qi Wang | Species: human | Application: WB - BIRC5 facilitates cisplatin-chemoresistance in a m6 A-dependent manner in ovarian cancer
Journal: Cancer Med | Authors: Yadan Fan | Species: human | Application: WB - METTL5 enhances the mRNA stability of TPRKB through m6A modification to facilitate the aggressive phenotypes of hepatocellular carcinoma cell
Journal: Exp Cell Res | Authors: Ming Luo | Species: human | Application: WB - The human 18S rRNA m6A methyltransferase METTL5 promotes tumorigenesis via DEPDC1 in lung squamous cell carcinoma
Journal: Front Oncol | Authors: Yang Yan | Species: human | Application: WB - METTL4 and METTL5 as biomarkers for recurrence-free survival in hepatocellular carcinoma patients
Journal: Future Oncol | Authors: Jialing Zhao | Species: human | Application: IHC - Loss of m6A Methyltransferase METTL5 Promotes Cardiac Hypertrophy Through Epitranscriptomic Control of SUZ12 Expression.
Journal: Front Cardiovasc Med | Authors: Yanchuang Han | Application: WB - METTL5 promotes gastric cancer progression via sphingomyelin metabolism
Journal: World J Gastrointest Oncol | Authors: Ya-Qiong Zhang | Species: human | Application: IHC - METTL5 Promotes Tumor Progression in Oral Squamous Cell Carcinoma by Activating the Myc Pathway
Journal: J Oral Pathol Med | Authors: Rong Huang | Species: human | Application: WB,IHC - Long noncoding RNA UBA6-AS1 inhibits the malignancy of ovarian cancer cells via suppressing the decay of UBA6 mRNA.
Journal: Bioengineered | Authors: Yaogang Wang | Species: human | Application: RIP - The potential role of methyltransferase-like 5 in deficient mismatch repair of uterine corpus endometrial carcinoma.
Journal: Bioengineered | Authors: Xiaojuan Liu | Species: human | Application: WB,IHC - Pathomics-based machine learning models for predicting METTL5 expression and prognosis in lung adenocarcinoma.
Journal: J Thorac Dis | Authors: Xu Chen
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