ACTL9 Polyclonal antibody 29885-1-AP proteintech
$149.00
In stock
SKU
29885-1-AP
| Catalog Number | 29885-1-AP |
| Synonyms | Actin like protein 9, Actin-like protein 9, ACTL7C, HSD21, hypothetical protein MGC33407 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF-P, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | mouse skeletal muscle tissue, rat skeletal muscle |
| Tested Applications — Positive IHC detected in | human liver tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF-P detected in | mouse testis tissue |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:2000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Positive WB detected in | mouse skeletal muscle tissue, rat skeletal muscle |
| Positive IHC detected in | human liver tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse testis tissue |
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag31805 Product name: Recombinant human MGC33407 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-172 aa of BC024028 Sequence: MDASRPKSSESQSSLEAPRPGPNPSPNVVNKPLQRDSPGMVADRLPPKTGAVVIDMGTGTCKVGFAGQASPTYTVATILGCQPKKPATSGQSGLQTFIGEAARVLPELTLVQPLRSGIVVDWDAAELIWRHLLEHDLRVATHDHPLLFSDPPFSPATNREKLVEVAFESLRS Predict reactive species |
| Full Name | hypothetical protein MGC33407 |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC024028 |
| Gene Symbol | ACTL9 |
| Gene ID (NCBI) | 284382 |
| RRID | AB_2923617 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TC94 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for ACTL9 antibody 29885-1-AP | Download protocol |
| IHC protocol for ACTL9 antibody 29885-1-AP | Download protocol |
| WB protocol for ACTL9 antibody 29885-1-AP | Download protocol |
| mouse | WB Ecotoxicol Environ Saf PFOA exposure decreases acrosome reaction and increases DNA oxidative damage by impairing the sperm head shaping in mice. Authors - Chuan Wang View Article |
| Citations | 1 |
| Dilutions | WB : 1:500-1:2000 IHC : 1:50-1:500 IF-P : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
ACTL9 (Actin Like 9), also named MGC33407, encodes a testis-specific actin-like protein that plays a role in fusion of proacrosomal vesicles and perinuclear theca formation (PMID: 33626338). ACTL9 protein contains 416 amino acids, which mainly localizes in the equatorial segment of human sperm head (PubMed: 33626338). Diseases associated with ACTL9 include spermatogenic failure and non-syndromic male infertility. Protocols Product Specific Protocols IF protocol for ACTL9 antibody 29885-1-AP Download protocol IHC protocol for ACTL9 antibody 29885-1-AP Download protocol WB protocol for ACTL9 antibody 29885-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- PFOA exposure decreases acrosome reaction and increases DNA oxidative damage by impairing the sperm head shaping in mice.
Journal: Ecotoxicol Environ Saf | Authors: Chuan Wang | Species: mouse | Application: WB
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