MGST2 Polyclonal antibody 25451-1-AP proteintech
$149.00
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SKU
25451-1-AP
| Catalog Number | 25451-1-AP |
| Synonyms | GST2, MGST II, Microsomal GST 2, Microsomal GST II |
| Host | Rabbit |
| Reactivity | human |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HUVEC cells, HeLa cells, HuH-7 cells |
| Tested Applications — Positive IP detected in | HuH-7 cells, HUVEC cells |
| Tested Applications — Positive IHC detected in | human liver tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:1000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Positive WB detected in | HUVEC cells, HeLa cells, HuH-7 cells |
| Positive IP detected in | HuH-7 cells, HUVEC cells |
| Positive IHC detected in | human liver tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Tested Reactivity | human |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag21105 Product name: Recombinant human MGST2 protein Source: e coli. -derived, PET30a Tag: 6*His Domain: 1-147 aa of BC025416 Sequence: MAGNSILLAAVSILSACQQSYFALQVGKARLKYKVTPPAVTGSPEFERVFRAQQNCVEFYPIFIITLWMAGWYFNQVFATCLGLVYIYGRHLYFWGYSEAAKKRITGFRLSLGILALLTLLGALGIANSFLDEYLDLNIAKKLRRQF Predict reactive species |
| Full Name | microsomal glutathione S-transferase 2 |
| Calculated Molecular Weight | 147 aa, 17 kDa |
| Observed Molecular Weight | 17 kDa |
| GenBank Accession Number | BC025416 |
| Gene Symbol | MGST2 |
| Gene ID (NCBI) | 4258 |
| RRID | AB_3669476 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99735 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for MGST2 antibody 25451-1-AP | Download protocol |
| IP protocol for MGST2 antibody 25451-1-AP | Download protocol |
| WB protocol for MGST2 antibody 25451-1-AP | Download protocol |
| Citations | - |
| Dilutions | WB : 1:500-1:1000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Microsomal glutathione S-transferase 2 (MGST2) is a 17 kDa trimeric integral membrane protein homologous to leukotriene C4 synthase (LTC4S) and catalyze the biosynthesis of the proinflammatory mediator leukotriene C4 (LTC4) in cells devoid of LTC4S (PMID: 23409838). Protocols Product Specific Protocols IHC protocol for MGST2 antibody 25451-1-AP Download protocol IP protocol for MGST2 antibody 25451-1-AP Download protocol WB protocol for MGST2 antibody 25451-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
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