MIOS Recombinant monoclonal antibody 83856-4-RR proteintech
$149.00
In stock
SKU
83856-4-RR
| Catalog Number | 83856-4-RR |
| Synonyms | 240832D12, GATOR2 complex protein MIOS, Missing oocyte meiosis regulator homolog |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | IHC, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | U2OS cells |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| Tested Reactivity | human, mouse |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Ag15148 Product name: Recombinant human MIOS protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 188-537 aa of BC005883 Sequence: AVALFNLDIRRAIQILNEGASSEKGDLNLNVVAMALSGYTDEKNSLWREMCSTLRLQLNNPYLCVMFAFLTSETGSYDGVLYENKVAVRDRVAFACKFLSDTQLNRYIEKLTNEMKEAGNLEGILLTGLTKDGVDLMESYVDRTGDVQTASYCMLQGSPLDVLKDERVQYWIENYRNLLDAWRFWHKRAEFDIHRSKLDPSSKPLAQVFVSCNFCGKSISYSCSAVPHQGRGFSQYGVSGSPTKSKVTSCPGCRKPLPRCALCLINMGTPVSSCPGGTKSDEKVDLSKDKKLAQFNNWFTWCHNCRHGGHAGHMLSWFRDHAECPVSACTCKCMQLDTTGNLVPAETVQP Predict reactive species |
| Full Name | missing oocyte, meiosis regulator, homolog (Drosophila) |
| Calculated Molecular Weight | 875 aa, 99 kDa |
| GenBank Accession Number | BC005883 |
| Gene Symbol | MIOS |
| Gene ID (NCBI) | 54468 |
| RRID | AB_3671440 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q9NXC5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for MIOS antibody 83856-4-RR | Download protocol |
| IHC protocol for MIOS antibody 83856-4-RR | Download protocol |
| Citations | - |
| Dilutions | IHC : 1:200-1:800 IF/ICC : 1:125-1:500 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 240832D12 |
| Conjugation | Unconjugated |
The mechanistic target of rapamycin complex 1 (mTORC1) controls growth by regulating anabolic and catabolic processes in response to environmental cues, including nutrients. Amino acids signal to mTORC1 through the Rag GTPases, which are regulated by several protein complexes, including GATOR1 and GATOR2. GATOR2, which has five components (WDR24, MIOS, WDR59, SEH1L, SEC13), is required for amino acids to activate mTORC1. PMID: 3583151 Protocols Product Specific Protocols IF protocol for MIOS antibody 83856-4-RR Download protocol IHC protocol for MIOS antibody 83856-4-RR Download protocol Standard Protocols Click here to view our Standard Protocols
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