MPG Polyclonal antibody 11481-2-AP proteintech
$149.00
In stock
SKU
11481-2-AP
| Catalog Number | 11481-2-AP |
| Synonyms | 3 alkyladenine DNA glycosylase, 3-alkyladenine DNA glycosylase, 3-methyladenine DNA glycosidase, AAG, ADPG |
| Host | Rabbit |
| Reactivity | human and More (1) |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HepG2 cells, Jurkat cells, L02 cells |
| Tested Applications — Positive IP detected in | HepG2 cells |
| Tested Applications — Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:1000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Positive WB detected in | HepG2 cells, Jurkat cells, L02 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag2034 Product name: Recombinant human MPG protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-293 aa of BC014991 Sequence: MPARSGAQFCRRMGQKKQRPARAGQPHSSSDAAQAPAEQPHSSSDAAQAPCPRERCLGPPTTPGPYRSIYFSSPKGHLTRLGLEFFDQPAVPLARAFLGQVLVRRLPNGTELRGRIVETEAYLGPEDEAAHSRGGRQTPRNRGMFMKPGTLYVYIIYGMYFCMNISSQGDGACVLLRALEPLEGLETMRQLRSTLRKGTASRVLKDRELCSGPSKLCQALAINKSFDQRDLAQDEAVWLERGPLEPSEPAVVAAARVGVGHAGEWARKPLRFYVRGSPWVSVVDRVAEQDTQA Predict reactive species |
| Full Name | N-methylpurine-DNA glycosylase |
| Calculated Molecular Weight | 293 aa, 32 kDa |
| Observed Molecular Weight | 33 kDa |
| GenBank Accession Number | BC014991 |
| Gene Symbol | MPG |
| Gene ID (NCBI) | 4350 |
| RRID | AB_2282144 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P29372 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for MPG antibody 11481-2-AP | Download protocol |
| IP protocol for MPG antibody 11481-2-AP | Download protocol |
| WB protocol for MPG antibody 11481-2-AP | Download protocol |
| human | WB Reprod Biomed Online Proteomic signature of menstrual blood mesenchymal stromal cells and their extracellular vesicles in women with unexplained infertility. Authors - Raminta Vaiciuleviciute View Article |
| mouse | WB J Radiat Res Abundance of BER-related proteins depends on cell proliferation status and the presence of DNA polymerase β. Authors - Mizuki Yamamoto View Article |
| Citations | 4 |
| Dilutions | WB : 1:500-1:1000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
N-methylpurine DNA glycosylase (MPG), an initiator glycosylase of the base excision repair (BER) pathway, typically repairs alkyl-induced DNA adducts, many of which are associated with genomic instability and tumorigenic risk [PMID:22801474] MPG initiates BER by removing TMZ-induced N3-methyladenine and N7-methylguanine base lesions, leaving abasic sites (AP sites) in DNA for further processing by BER [PMID:21377995]. MPG involves in modulating the genotoxic threshold response induced by the Sn2 alkylating agent ethyl methanesulfonate (EMS) [PMID:21071726]. Protocols Product Specific Protocols IHC protocol for MPG antibody 11481-2-AP Download protocol IP protocol for MPG antibody 11481-2-AP Download protocol WB protocol for MPG antibody 11481-2-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- LncRNA GUARDIN suppresses cellular senescence through a LRP130-PGC1α-FOXO4-p21-dependent signaling axis.
Journal: EMBO Rep | Authors: Xuedan Sun | Species: human | Application: WB - A Novel Pseudogene Methylation Signature to Predict Temozolomide Outcome in Non-G-CIMP Glioblastomas.
Journal: J Oncol | Authors: Bowen Li | Species: human | Application: WB - Abundance of BER-related proteins depends on cell proliferation status and the presence of DNA polymerase β.
Journal: J Radiat Res | Authors: Mizuki Yamamoto | Species: mouse | Application: WB - Proteomic signature of menstrual blood mesenchymal stromal cells and their extracellular vesicles in women with unexplained infertility.
Journal: Reprod Biomed Online | Authors: Raminta Vaiciuleviciute | Species: human | Application: WB
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