MSH2 Recombinant monoclonal antibody 82775-1-RR proteintech
$149.00
In stock
SKU
82775-1-RR
| Catalog Number | 82775-1-RR |
| Synonyms | MSH 2 |
| Host | Rabbit |
| Reactivity | human |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HeLa cells, HepG2 cells, Caco-2 cells, A549 cells, HCT 116 cells |
| Tested Applications — Positive IHC detected in | human colon tissue, human colon cancer, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HeLa cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Positive WB detected in | HeLa cells, HepG2 cells, Caco-2 cells, A549 cells, HCT 116 cells |
| Positive IHC detected in | human colon tissue, human colon cancer, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Tested Reactivity | human |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Ag7835 Product name: Recombinant human MSH2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 585-934 aa of BC021566 Sequence: SSGYVEPMQTLNDVLAQLDAVVSFAHVSNGAPVPYVRPAILEKGQGRIILKASRHACVEVQDEIAFIPNDVYFEKDKQMFHIITGPNMGGKSTYIRQTGVIVLMAQIGCFVPCESAEVSIVDCILARVGAGDSQLKGVSTFMAEMLETASILRSATKDSLIIIDELGRGTSTYDGFGLAWAISEYIATKIGAFCMFATHFHELTALANQIPTVNNLHVTALTTEETLTMLYQVKKGVCDQSFGIHVAELANFPKHVIECAKQKALELEEFQYIGESQGYDIMEPAAKKCYLEREQGEKIIQEFLSKVKQMPFTEMSEENITIKLKQLKAEVIAKNNSFVNEIISRIKVTT Predict reactive species |
| Full Name | mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli) |
| Calculated Molecular Weight | 105 kDa |
| Observed Molecular Weight | 100 kDa-105 kDa |
| GenBank Accession Number | BC021566 |
| Gene Symbol | MSH2 |
| Gene ID (NCBI) | 4436 |
| RRID | AB_3086533 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | P43246 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for MSH2 antibody 82775-1-RR | Download protocol |
| IHC protocol for MSH2 antibody 82775-1-RR | Download protocol |
| WB protocol for MSH2 antibody 82775-1-RR | Download protocol |
| Citations | - |
| Dilutions | WB : 1:5000-1:50000 IHC : 1:1000-1:4000 IF/ICC : 1:400-1:1600 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 6O4 |
| Conjugation | Unconjugated |
MSH2, named for its homologous to the E.coli MutS gene, takes part in DNA mismatch repair(MMR) to maintain the stability of DNA. MSH2 contains a helix-turn-helix domain, which responds for binding to DNA. Also, MSH2 has a direct role in mutation avoidance and micro-satellite stability in cell. Protocols Product Specific Protocols IF protocol for MSH2 antibody 82775-1-RR Download protocol IHC protocol for MSH2 antibody 82775-1-RR Download protocol WB protocol for MSH2 antibody 82775-1-RR Download protocol Standard Protocols Click here to view our Standard Protocols
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