MUS81 Polyclonal antibody 11018-1-AP proteintech
$149.00
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SKU
11018-1-AP
| Catalog Number | 11018-1-AP |
| Synonyms | EC:3.1.22.- |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, ELISA |
| Applications | WB, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HepG2 cells, NIH/3T3 cells |
| Tested Applications — Positive IF/ICC detected in | MCF-7 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:2000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| Positive WB detected in | HepG2 cells, NIH/3T3 cells |
| Positive IF/ICC detected in | MCF-7 cells |
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag1476 Product name: Recombinant human MUS81 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 202-551 aa of BC009999 Sequence: RYSLTPEGLELAQKLAESEGLSLLNVGIGPKEPPGEETAVPGAASAELASEAGVQQQPLELRPGEYRVLLCVDIGETRGGGHRPELLRELQRLHVTHTVRKLHVGDFVWVAQETNPRDPANPGELVLDHIVERKRLDDLCSSIIDGRFREQKFRLKRCGLERRVYLVEEHGSVHNLSLPESTLLQAVTNTQVIDGFFVKRTADIKESAAYLALLTRGLQRLYQGHTLRSRPWGTPGNPESGAMTSPNPLCSLLTFSDFNAGAIKNKAQSVREVFARQLMQVRGVSGEKAAALVDRYSTPASLLAAYDACATPKEQETLLSTIKCGRLQRNLGPALSRTLSQLYCSYGPLT Predict reactive species |
| Full Name | MUS81 endonuclease homolog (S. cerevisiae) |
| Calculated Molecular Weight | 61 kDa |
| Observed Molecular Weight | 52-72 kDa |
| GenBank Accession Number | BC009999 |
| Gene Symbol | MUS81 |
| Gene ID (NCBI) | 80198 |
| RRID | AB_2147130 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96NY9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for MUS81 antibody 11018-1-AP | Download protocol |
| WB protocol for MUS81 antibody 11018-1-AP | Download protocol |
| human | IF Am J Transl Res MUS81 inhibits cell proliferation and migration in breast cancer by promoting the expression of CDKN2A(p16(INK4a)). Authors - Rong Xie KD Validated View Article |
| Arnaud (Verified Customer) (09-26-2025) | 2 bands at 70kDa, work well at 1:1000 dilution Applications: Western Blot Primary Antibody Dilution: 1:1000 for WB Cell Tissue Type: HeLa |
| Citations | 3 |
| Dilutions | WB : 1:500-1:2000 IF/ICC : 1:20-1:200 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
The Crossover junction endonuclease MUS81 has associated endonuclease activity against structure-specific oligonucleotide substrates, including synthetic Holliday junctions(PMID:11741546). It interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. The open reading frame of human MUS81 predicts a translation product of 551 amino acids with a molecular mass of 59 kD. There are some reports showed that the enzyme can express a 72 kDa protein in human tissues(PMID:12724407). Protocols Product Specific Protocols IF protocol for MUS81 antibody 11018-1-AP Download protocol WB protocol for MUS81 antibody 11018-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- EMSY inhibits homologous recombination repair and the interferon response, promoting lung cancer immune evasion.
Journal: Cell | Authors: Antonio Marzio KD Validated | Species: human | Application: WB,IHC - 53BP1 and BRCA1 control pathway choice for stalled replication restart.
Journal: Elife | Authors: Yixi Xu | Application: WB - MUS81 inhibits cell proliferation and migration in breast cancer by promoting the expression of CDKN2A(p16(INK4a)).
Journal: Am J Transl Res | Authors: Rong Xie KD Validated | Species: human | Application: IF
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