MYBBP1A Monoclonal antibody 67996-1-Ig proteintech
$149.00
In stock
SKU
67996-1-Ig
| Catalog Number | 67996-1-Ig |
| Synonyms | 1H2D10, Myb binding protein 1A, Myb-binding protein 1A, PAP2 |
| Host | Mouse |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, CoIP, ELISA |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Mouse / IgG1 |
| Tested Applications — Positive WB detected in | LNCaP cells, 4T1 cells, HEK-293 cells, HSC-T6 cells, NIH/3T3 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells |
| Tested Applications — Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HepG2 cells, NIH/3T3 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| Positive WB detected in | LNCaP cells, 4T1 cells, HEK-293 cells, HSC-T6 cells, NIH/3T3 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, NIH/3T3 cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag6008 Product name: Recombinant human MYBBP1A protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 979-1328 aa of BC050546 Sequence: STALSSFLTKRNSPLTVPMFLSLFSRHPVLCQSLLPILVQHITGPVRPRRQACLLLQKTLSMREVRSCFEDPEWKQLMGQVLAKVTENLRVLGEAQTKAQHQQALSSLELLNVLFRTCKHEKLTLDLTVLLGVLQGQQQSLQQGAHSTGSSRLHDLYWQAMKTLGVQRPKLEKKDAKEIPSATQSPISKKRKKKGFLPETKKRKKRKSEDGTPAEDGTPAATGGSQPPSMGRKKRNRTKAKVPAQANGTPTTKSPAPGAPTRSPSTPAKSPKLQKKNQKPSQVNGAPGSPTEPAGQKQHQKALPKKGVLGKSPLSALARKKARLSLVIRSPSLLQSGAKKKAQVRKAGKP Predict reactive species |
| Full Name | MYB binding protein (P160) 1a |
| Calculated Molecular Weight | 149 kDa |
| Observed Molecular Weight | 140 kDa |
| GenBank Accession Number | BC050546 |
| Gene Symbol | MYBBP1A |
| Gene ID (NCBI) | 10514 |
| RRID | AB_2918745 |
| Conjugate | Unconjugated |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9BQG0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for MYBBP1A antibody 67996-1-Ig | Download protocol |
| IHC protocol for MYBBP1A antibody 67996-1-Ig | Download protocol |
| WB protocol for MYBBP1A antibody 67996-1-Ig | Download protocol |
| human | WB,CoIP Nat Cancer METTL3-based epitranscriptomic editing screening identifies functional m(6)A sites in cancers. Authors - Xin Xu View Article |
| Citations | 1 |
| Dilutions | WB : 1:5000-1:50000 IHC : 1:500-1:2000 IF/ICC : 1:300-1:1200 |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Clone Number | 1H2D10 |
| Conjugation | Unconjugated |
The protooncogene MYB is predominantly expressed in immature hemopoietic cells where it has an essential role in hemopoietic cell proliferation and differentiation. Oncogenically activated forms of MYB is generally N- and/or C-terminal truncations of the normal MYB protein. Removal of the C terminus of MYB disrupts or deletes a region termed the negative regulatory domain (NRD), resulting in an increase in DNA binding, transactivation, and transformation by MYB. One feature of the NRD is a leucine zipper-like motif [PMID: 8302594]. Murine Myb-binding protein-1a (MYBBP1A), originally called P160, was identified by its ability to interact specifically with Myb via this leucine zipper-like motif. MYBBP1A modulates MYB activity upon binding to the MYB NRD [PMID: 10644447, 9447996]. Protocols Product Specific Protocols IF protocol for MYBBP1A antibody 67996-1-Ig Download protocol IHC protocol for MYBBP1A antibody 67996-1-Ig Download protocol WB protocol for MYBBP1A antibody 67996-1-Ig Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- METTL3-based epitranscriptomic editing screening identifies functional m(6)A sites in cancers.
Journal: Nat Cancer | Authors: Xin Xu | Species: human | Application: WB,CoIP
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